Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1763921..1764581 Replicon chromosome
Accession NZ_CP077823
Organism Klebsiella africana strain FF1019

Toxin (Protein)


Gene name higB Uniprot ID A0A8B6IX92
Locus tag KU660_RS08460 Protein ID WP_008804168.1
Coordinates 1764228..1764581 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7U2ZUJ7
Locus tag KU660_RS08455 Protein ID WP_061153979.1
Coordinates 1763921..1764223 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU660_RS08410 (1758977) 1758977..1759534 + 558 Protein_1647 hypothetical protein -
KU660_RS08415 (1759893) 1759893..1760357 + 465 WP_251354983.1 hypothetical protein -
KU660_RS08420 (1760354) 1760354..1760560 + 207 WP_251354984.1 helix-turn-helix transcriptional regulator -
KU660_RS08425 (1760895) 1760895..1761152 + 258 WP_000288812.1 antitoxin -
KU660_RS08430 (1761153) 1761153..1761485 + 333 WP_040245064.1 type II toxin-antitoxin system PemK/MazF family toxin -
KU660_RS08440 (1761807) 1761807..1763243 + 1437 WP_251354986.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
KU660_RS08450 (1763569) 1763569..1763718 - 150 Protein_1653 antitoxin -
KU660_RS08455 (1763921) 1763921..1764223 - 303 WP_061153979.1 XRE family transcriptional regulator Antitoxin
KU660_RS08460 (1764228) 1764228..1764581 - 354 WP_008804168.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KU660_RS08465 (1764994) 1764994..1765452 + 459 WP_136032098.1 hypothetical protein -
KU660_RS08470 (1765541) 1765541..1766995 - 1455 WP_203033938.1 AMP nucleosidase -
KU660_RS08475 (1767126) 1767126..1767371 - 246 WP_136032102.1 signal transduction protein PmrD -
KU660_RS08480 (1767642) 1767642..1769264 + 1623 WP_136032104.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(29-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13528.51 Da        Isoelectric Point: 10.1729

>T208011 WP_008804168.1 NZ_CP077823:c1764581-1764228 [Klebsiella africana]
VWTIKTTDMFDRWFTSLNDIDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRVQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYHEMLAVADREFTNWLNRLKEKE

Download         Length: 354 bp

>T208011 NZ_CP103557:4799184-4799561 [Escherichia coli O25b:H4-ST131]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11135.94 Da        Isoelectric Point: 5.1976

>AT208011 WP_061153979.1 NZ_CP077823:c1764223-1763921 [Klebsiella africana]
MGRTLEQLLIDEKPDVVAEAQAIAKDILLNIHLAELREKVQKTQVEMAQDLGIKQPTVAGMEKPGRDLKLSTLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT208011 NZ_CP103557:4798760-4799137 [Escherichia coli O25b:H4-ST131]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6IX92

Antitoxin

Source ID Structure
AlphaFold DB A0A7U2ZUJ7

References