Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3223964..3224691 Replicon chromosome
Accession NC_004337
Organism Shigella flexneri 2a str. 301

Toxin (Protein)


Gene name higB Uniprot ID P64580
Locus tag SF3123 Protein ID NP_708888.2
Coordinates 3224377..3224691 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID P67703
Locus tag SF3122 Protein ID NP_708887.1
Coordinates 3223964..3224380 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF3120 3219324..3221675 + 2352 NP_708885.1 alpha-glucosidase -
SF3121 3221901..3223919 + 2019 NP_708886.1 NADPH dehydrogenase -
SF3122 3223964..3224380 - 417 NP_708887.1 hypothetical protein Antitoxin
SF3123 3224377..3224691 - 315 NP_708888.2 hypothetical protein Toxin
SF3124 3224976..3226112 - 1137 NP_708889.1 SAM-dependent 23S ribosomal RNA mG1835 methyltransferase -
SF3125 3226161..3226700 + 540 NP_708890.1 hypothetical protein -
SF3126 3226777..3227469 + 693 NP_708891.1 hypothetical protein -
SF3127 3227530..3228546 + 1017 NP_708892.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T20797 NP_708888.2 NC_004337:c3224691-3224377 [Shigella flexneri 2a str. 301]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T20797 NC_004337:c3224691-3224377 [Shigella flexneri 2a str. 301]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT20797 NP_708887.1 NC_004337:c3224380-3223964 [Shigella flexneri 2a str. 301]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT20797 NC_004337:c3224380-3223964 [Shigella flexneri 2a str. 301]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5YCL

Antitoxin

Source ID Structure
PDB 6IRP
PDB 5YCL
AlphaFold DB P67703

References