Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4328413..4329059 Replicon chromosome
Accession NZ_CP077810
Organism Klebsiella pneumoniae strain FF904

Toxin (Protein)


Gene name higB Uniprot ID A0A486UPU4
Locus tag KU662_RS20860 Protein ID WP_032417639.1
Coordinates 4328413..4328760 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag KU662_RS20865 Protein ID WP_002920557.1
Coordinates 4328760..4329059 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU662_RS20850 (4324339) 4324339..4325772 + 1434 WP_002920564.1 glycogen synthase GlgA -
KU662_RS20855 (4325790) 4325790..4328237 + 2448 WP_002920561.1 glycogen phosphorylase -
KU662_RS20860 (4328413) 4328413..4328760 + 348 WP_032417639.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KU662_RS20865 (4328760) 4328760..4329059 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
KU662_RS20870 (4329122) 4329122..4330630 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KU662_RS20875 (4330835) 4330835..4331164 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KU662_RS20880 (4331215) 4331215..4332045 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
KU662_RS20885 (4332095) 4332095..4332853 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13527.52 Da        Isoelectric Point: 5.6802

>T207907 WP_032417639.1 NZ_CP077810:4328413-4328760 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFEPLRKAI
VLCAGDKDGMNEKRFYKELITLADREFSQHLTKER

Download         Length: 348 bp

>T207907 NZ_CP103540:1809981-1810232 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT207907 WP_002920557.1 NZ_CP077810:4328760-4329059 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT207907 NZ_CP103540:1810229-1810483 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATTATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486UPU4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References