Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4302224..4302810 Replicon chromosome
Accession NZ_CP077810
Organism Klebsiella pneumoniae strain FF904

Toxin (Protein)


Gene name doc Uniprot ID A0A485R3H6
Locus tag KU662_RS20750 Protein ID WP_032417643.1
Coordinates 4302442..4302810 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KU662_RS20745 Protein ID WP_004174006.1
Coordinates 4302224..4302445 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU662_RS20725 (4298381) 4298381..4299307 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KU662_RS20730 (4299304) 4299304..4300581 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KU662_RS20735 (4300578) 4300578..4301345 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KU662_RS20740 (4301347) 4301347..4302060 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KU662_RS20745 (4302224) 4302224..4302445 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KU662_RS20750 (4302442) 4302442..4302810 + 369 WP_032417643.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KU662_RS20755 (4303083) 4303083..4304399 + 1317 WP_032417642.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KU662_RS20760 (4304506) 4304506..4305393 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KU662_RS20765 (4305390) 4305390..4306235 + 846 WP_032416348.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KU662_RS20770 (4306237) 4306237..4307307 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4299304..4308044 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13584.98 Da        Isoelectric Point: 8.6410

>T207906 WP_032417643.1 NZ_CP077810:4302442-4302810 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRMLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T207906 NZ_CP103540:998524-998859 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT207906 WP_004174006.1 NZ_CP077810:4302224-4302445 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT207906 NZ_CP103540:998276-998524 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A485R3H6

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References