Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4332001..4332647 Replicon chromosome
Accession NZ_CP077806
Organism Klebsiella pneumoniae strain FF979

Toxin (Protein)


Gene name higB Uniprot ID A0A483JFR4
Locus tag KU665_RS20570 Protein ID WP_004188313.1
Coordinates 4332001..4332348 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag KU665_RS20575 Protein ID WP_002920557.1
Coordinates 4332348..4332647 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU665_RS20560 (KU665_20515) 4327927..4329360 + 1434 WP_002920564.1 glycogen synthase GlgA -
KU665_RS20565 (KU665_20520) 4329378..4331825 + 2448 WP_002920561.1 glycogen phosphorylase -
KU665_RS20570 (KU665_20525) 4332001..4332348 + 348 WP_004188313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KU665_RS20575 (KU665_20530) 4332348..4332647 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
KU665_RS20580 (KU665_20535) 4332710..4334218 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KU665_RS20585 (KU665_20540) 4334423..4334752 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KU665_RS20590 (KU665_20545) 4334803..4335633 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
KU665_RS20595 (KU665_20550) 4335683..4336441 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13548.57 Da        Isoelectric Point: 6.2327

>T207869 WP_004188313.1 NZ_CP077806:4332001-4332348 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T207869 NZ_CP103529:3648363-3648581 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT207869 WP_002920557.1 NZ_CP077806:4332348-4332647 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT207869 NZ_CP103529:3647963-3648337 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483JFR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References