Detailed information of TA system TA207851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4686746..4687343
• Replicon: chromosome
• Accession: NZ_CP077805
• Organism: Klebsiella variicola strain FF907

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: KU664_RS22850
• Protein ID: WP_012542526.1
• Coordinates: 4687026..4687343 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KU664_RS22845
• Protein ID: WP_012542525.1
• Coordinates: 4686746..4687033 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU664_RS22815 (KU664_22745)	4682606..4682854	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
KU664_RS22820 (KU664_22750)	4682871..4683212	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KU664_RS22825 (KU664_22755)	4683243..4684358	-	1116	WP_162499993.1	MBL fold metallo-hydrolase	-
KU664_RS22830 (KU664_22760)	4684538..4685119	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
KU664_RS22835 (KU664_22765)	4685119..4685487	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
KU664_RS22840 (KU664_22770)	4685607..4686260	+	654	WP_012542524.1	oxygen-insensitive NAD(P)H nitroreductase	-
KU664_RS22845 (KU664_22775)	4686746..4687033	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
KU664_RS22850 (KU664_22780)	4687026..4687343	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KU664_RS22855 (KU664_22785)	4687528..4688571	-	1044	WP_064170485.1	DUF2157 domain-containing protein	-
KU664_RS22860 (KU664_22790)	4689036..4689902	-	867	WP_022065427.1	helix-turn-helix transcriptional regulator	-
KU664_RS22865 (KU664_22795)	4690011..4691438	+	1428	WP_023297179.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T207851 WP_012542526.1 NZ_CP077805:c4687343-4687026 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T207851 NZ_CP103523:80982-81287 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT207851 WP_012542525.1 NZ_CP077805:c4687033-4686746 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT207851 NZ_CP103523:80762-80980 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2