Detailed information of TA system TA207828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1255826..1256439
• Replicon: chromosome
• Accession: NZ_CP077803
• Organism: Rhodospirillum rubrum strain DSM 467

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q2RVI0
• Locus tag: KUL73_RS05545
• Protein ID: WP_011388819.1
• Coordinates: 1255826..1256122 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KUL73_RS05550
• Protein ID: WP_014626089.1
• Coordinates: 1256131..1256439 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUL73_RS05505 (KUL73_05505)	1251047..1251229	+	183	WP_014626086.1	DUF1192 family protein	-
KUL73_RS05510 (KUL73_05510)	1251328..1252107	+	780	WP_011388812.1	3-hydroxybutyrate dehydrogenase	-
KUL73_RS05515 (KUL73_05515)	1252117..1252572	+	456	WP_011388813.1	thioesterase family protein	-
KUL73_RS05520 (KUL73_05520)	1252576..1253559	-	984	WP_011388814.1	aldo/keto reductase	-
KUL73_RS05525 (KUL73_05525)	1253665..1254600	+	936	WP_011388815.1	LysR family transcriptional regulator	-
KUL73_RS05530 (KUL73_05530)	1254695..1254910	+	216	WP_011388816.1	ribbon-helix-helix domain-containing protein	-
KUL73_RS05535 (KUL73_05535)	1254904..1255269	+	366	WP_011388817.1	type II toxin-antitoxin system VapC family toxin	-
KUL73_RS05540 (KUL73_05540)	1255307..1255651	+	345	WP_011388818.1	LysR substrate-binding domain-containing protein	-
KUL73_RS05545 (KUL73_05545)	1255826..1256122	+	297	WP_011388819.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUL73_RS05550 (KUL73_05550)	1256131..1256439	+	309	WP_014626089.1	HigA family addiction module antitoxin	Antitoxin
KUL73_RS05555 (KUL73_05555)	1256594..1257379	+	786	WP_011388821.1	ATP-binding cassette domain-containing protein	-
KUL73_RS05560 (KUL73_05560)	1257376..1258077	+	702	WP_011388822.1	GTP-binding protein	-
KUL73_RS05565 (KUL73_05565)	1258079..1259260	+	1182	WP_011388823.1	cysteine desulfurase family protein	-
KUL73_RS05570 (KUL73_05570)	1259304..1260029	+	726	WP_011388824.1	(Fe-S)-binding protein	-
KUL73_RS05575 (KUL73_05575)	1260133..1260603	-	471	WP_011388825.1	Hsp20 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11623.24 Da
• Isoelectric Point: 6.4797

>T207828 WP_011388819.1 NZ_CP077803:1255826-1256122 [Rhodospirillum rubrum]
MIVGFRDEWLRAFFVEDIHCRSIPADLESRLFRKLQMIDDAMTDQDLRVPPSNHFEKLHGILDGLYSIRVNKQWRLIFRW
DGGRGEASGIYLDDHGYR

Nucleotide

• Length: 297 bp

>T207828 NZ_CP103521:1112628-1112939 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11509.47 Da
• Isoelectric Point: 10.5835

>AT207828 WP_014626089.1 NZ_CP077803:1256131-1256439 [Rhodospirillum rubrum]
MMMTKRKPVTVGEILVEEFMGPLGLTQAALAEAMGVQRKHVNELCNNRRTVTAATALILARVFGNSPDFWLNAQRRNDLW
EVLHSPKDVERIERAKPLALRA

Nucleotide

• Length: 309 bp

>AT207828 NZ_CP103521:1112936-1113355 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: Q2RVI0