Detailed information of TA system TA020782

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1397783..1398008
• Replicon: chromosome
• Accession: NC_004337
• Organism: Shigella flexneri 2a str. 301

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: SF1350a
• Protein ID: YP_008439244.1
• Coordinates: 1397796..1398008 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1397783..1397841 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF1343	1392892..1393302	-	411	NP_707240.3	IS2 repressor TnpA	-
SF1344	1394096..1394842	+	747	NP_707241.4	DnaC-like chromosome replication protein	-
SF1345	1394857..1395279	+	423	NP_707242.1	bacteriophage protein	-
SF1346	1395280..1395693	+	414	NP_707243.1	bacteriophage protein	-
SF1347	1395786..1396004	+	219	NP_707244.1	hypothetical protein	-
SF1348	1396006..1396371	+	366	NP_707245.1	hypothetical protein	-
SF1349	1396368..1397033	+	666	NP_707246.1	hypothetical protein	-
SF1350	1397033..1397398	+	366	NP_707247.1	hypothetical protein	-
-	1397783..1397841	-	59	-	-	Antitoxin
SF1350a	1397796..1398008	+	213	YP_008439244.1	regulatory protein	Toxin
SF1350b	1398245..1398496	+	252	YP_008439245.1	hypothetical protein	-
SF1351	1398514..1398909	-	396	NP_707248.1	insertion element IS1 protein InsB	-
SF1352	1398936..1399211	-	276	NP_707249.1	insertion element IS1 protein InsA	-
SF1353	1399345..1399944	+	600	NP_707250.1	bacteriophage protein	-
SF1354	1399944..1400234	+	291	NP_707251.1	bacteriophage protein	-
SF1355	1400231..1400785	+	555	NP_707252.1	bacteriophage protein	-
SF1356	1401173..1401475	+	303	NP_707253.1	insertion sequence element IS600 transposase	-
SF1357	1401511..1402329	+	819	NP_707254.1	insertion sequence element IS600 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1385770..1444799	59029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7880.44 Da
• Isoelectric Point: 9.2663

>T20782 YP_008439244.1 NC_004337:1397796-1398008 [Shigella flexneri 2a str. 301]
MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T20782 NC_004337:1397796-1398008 [Shigella flexneri 2a str. 301]
ATGTCTCACAAATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT20782 NC_004337:c1397841-1397783 [Shigella flexneri 2a str. 301]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Antitoxin