Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324078..324664 Replicon chromosome
Accession NZ_CP077779
Organism Klebsiella pneumoniae strain CDI231

Toxin (Protein)


Gene name doc Uniprot ID A0A9J6Y968
Locus tag KUM55_RS01510 Protein ID WP_025367836.1
Coordinates 324296..324664 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KUM55_RS01505 Protein ID WP_004174006.1
Coordinates 324078..324299 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUM55_RS01485 (KUM55_01485) 320235..321161 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KUM55_RS01490 (KUM55_01490) 321158..322435 + 1278 WP_004188292.1 branched chain amino acid ABC transporter permease LivM -
KUM55_RS01495 (KUM55_01495) 322432..323199 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KUM55_RS01500 (KUM55_01500) 323201..323914 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KUM55_RS01505 (KUM55_01505) 324078..324299 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KUM55_RS01510 (KUM55_01510) 324296..324664 + 369 WP_025367836.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KUM55_RS01515 (KUM55_01515) 324937..326253 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KUM55_RS01520 (KUM55_01520) 326360..327247 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KUM55_RS01525 (KUM55_01525) 327244..328089 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KUM55_RS01530 (KUM55_01530) 328091..329161 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 321158..329898 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13449.86 Da        Isoelectric Point: 7.2790

>T207799 WP_025367836.1 NZ_CP077779:324296-324664 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIECEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLLG

Download         Length: 369 bp

>T207799 NZ_CP103515:1321989-1322387 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT207799 WP_004174006.1 NZ_CP077779:324078-324299 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT207799 NZ_CP103515:1321762-1321989 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References