Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 60053..60780 | Replicon | plasmid pCDI694-216.6 |
| Accession | NZ_CP077778 | ||
| Organism | Klebsiella pneumoniae strain CDI694 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | KUM43_RS28805 | Protein ID | WP_011251285.1 |
| Coordinates | 60469..60780 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | KUM43_RS28800 | Protein ID | WP_011251286.1 |
| Coordinates | 60053..60472 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KUM43_RS28770 (KUM43_28765) | 55224..55694 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| KUM43_RS28775 (KUM43_28770) | 56007..56642 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| KUM43_RS28780 (KUM43_28775) | 57058..57678 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| KUM43_RS28785 (KUM43_28780) | 57699..58487 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| KUM43_RS28790 (KUM43_28785) | 58501..58866 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| KUM43_RS28795 (KUM43_28790) | 58938..59906 | - | 969 | WP_074428168.1 | IS5 family transposase | - |
| KUM43_RS28800 (KUM43_28795) | 60053..60472 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| KUM43_RS28805 (KUM43_28800) | 60469..60780 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| KUM43_RS28810 (KUM43_28805) | 60985..61422 | - | 438 | Protein_61 | DDE-type integrase/transposase/recombinase | - |
| KUM43_RS28815 (KUM43_28810) | 61557..62254 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| KUM43_RS28820 (KUM43_28815) | 62256..62705 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| KUM43_RS28825 (KUM43_28820) | 62722..63033 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| KUM43_RS28830 (KUM43_28825) | 63047..63388 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| KUM43_RS28835 (KUM43_28830) | 63448..64404 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| KUM43_RS28840 (KUM43_28835) | 64829..65269 | - | 441 | WP_011251275.1 | hypothetical protein | - |
| KUM43_RS28845 (KUM43_28840) | 65275..65769 | - | 495 | WP_217800589.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..216645 | 216645 | |
| - | inside | IScluster/Tn | - | - | 43602..67689 | 24087 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T207796 WP_011251285.1 NZ_CP077778:c60780-60469 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T207796 NZ_CP103515:c604098-603634 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT207796 WP_011251286.1 NZ_CP077778:c60472-60053 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT207796 NZ_CP103515:c604433-604098 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|