Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 416621..417239 Replicon chromosome
Accession NC_004337
Organism Shigella flexneri 2a str. 301

Toxin (Protein)


Gene name Hha Uniprot ID P0ACE6
Locus tag SF0405 Protein ID NP_706353.2
Coordinates 416621..416839 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A384KCZ5
Locus tag SF0406 Protein ID NP_706354.1
Coordinates 416865..417239 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF0400 412513..412824 - 312 NP_706349.2 hypothetical protein -
SF0401 413203..413556 + 354 NP_706350.1 hypothetical protein -
SF0402 413598..415148 - 1551 NP_706351.2 hypothetical protein -
SF0403 415312..415782 - 471 NP_706352.2 hypothetical protein -
SF0405 416621..416839 - 219 NP_706353.2 gene expression modulator Toxin
SF0406 416865..417239 - 375 NP_706354.1 Hha toxicity attenuator Antitoxin
SF0407 417785..420934 - 3150 NP_706355.1 multidrug efflux system protein AcrB -
SF0408 420957..422150 - 1194 NP_706356.2 multidrug efflux system transporter AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 411155..412483 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T20777 NP_706353.2 NC_004337:c416839-416621 [Shigella flexneri 2a str. 301]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T20777 NC_004337:c416839-416621 [Shigella flexneri 2a str. 301]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT20777 NP_706354.1 NC_004337:c417239-416865 [Shigella flexneri 2a str. 301]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT20777 NC_004337:c417239-416865 [Shigella flexneri 2a str. 301]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0ACE6

Antitoxin

Source ID Structure
AlphaFold DB A0A384KCZ5

References