Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 299410..300104 Replicon chromosome
Accession NC_004337
Organism Shigella flexneri 2a str. 301

Toxin (Protein)


Gene name yafO Uniprot ID A0A0H2UYJ8
Locus tag SF0281 Protein ID NP_706233.1
Coordinates 299706..300104 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID A0A384KES1
Locus tag SF0280 Protein ID NP_706232.1
Coordinates 299410..299703 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF0276 295049..295546 + 498 NP_706228.1 hypothetical protein -
SF0278 295763..297502 - 1740 NP_706229.1 flagellar biosynthetic protein FlhA -
SF0277 297447..298232 + 786 NP_706230.2 hypothetical protein -
SF0279 298303..299358 + 1056 NP_706231.1 DNA polymerase IV -
SF0280 299410..299703 + 294 NP_706232.1 antitoxin of the YafO-YafN toxin-antitoxin system Antitoxin
SF0281 299706..300104 + 399 NP_706233.1 toxin YafO Toxin
SF0282 300114..300566 + 453 NP_706234.1 acyltransferase -
SF0283 300812..301855 + 1044 NP_706235.2 hypothetical protein -
SF0285 302588..304045 - 1458 NP_706237.1 aminoacyl-histidine dipeptidase -
SF0286 304307..304765 + 459 NP_706238.1 xanthine-guanine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrA / gtrB / gtrII 298303..339701 41398


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T20776 NP_706233.1 NC_004337:299706-300104 [Shigella flexneri 2a str. 301]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T20776 NC_004337:299706-300104 [Shigella flexneri 2a str. 301]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT20776 NP_706232.1 NC_004337:299410-299703 [Shigella flexneri 2a str. 301]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT20776 NC_004337:299410-299703 [Shigella flexneri 2a str. 301]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2UYJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A384KES1

References