Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 4514457..4515111 Replicon chromosome
Accession NC_004129
Organism Pseudomonas protegens Pf-5

Toxin (Protein)


Gene name higB Uniprot ID Q4K9S3
Locus tag PFL_RS19875 Protein ID WP_011062197.1
Coordinates 4514457..4514807 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q4K9S2
Locus tag PFL_RS19880 Protein ID WP_011062198.1
Coordinates 4514794..4515111 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PFL_RS19865 4511216..4512748 + 1533 WP_011062195.1 NADH-quinone oxidoreductase subunit M -
PFL_RS19870 4512756..4514219 + 1464 WP_011062196.1 NADH-quinone oxidoreductase subunit NuoN -
PFL_RS19875 4514457..4514807 + 351 WP_011062197.1 hypothetical protein Toxin
PFL_RS19880 4514794..4515111 + 318 WP_011062198.1 transcriptional regulator Antitoxin
PFL_RS19885 4515180..4516562 - 1383 WP_011062199.1 cysteine--tRNA ligase -
PFL_RS19890 4516581..4518281 - 1701 WP_011062200.1 glutamine--tRNA ligase/YqeY domain fusion protein -
PFL_RS19895 4518552..4519055 + 504 WP_011062201.1 peptidyl-prolyl cis-trans isomerase -
PFL_RS19900 4519052..4519804 + 753 WP_011062202.1 UDP-2,3-diacylglucosamine diphosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4341380..4590949 249569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(48-101)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13922.05 Da        Isoelectric Point: 9.6226

>T20769 WP_011062197.1 NC_004129:4514457-4514807 [Pseudomonas protegens Pf-5]
MNALFIELPAFARYRKGFLDDELFCELQIELLRHPHAGVLIQGTGGLRKMRFVDERRNKGRRGGLRVIYYWWSGGAQFWL
FTLFDKEQMDDLLPQQRQQLKQLLEREIEARRHDQT

Download         Length: 351 bp

>T20769 NC_004129:4514457-4514807 [Pseudomonas protegens Pf-5]
ATGAATGCTCTGTTTATTGAACTACCGGCATTTGCGCGATACCGAAAAGGCTTCCTTGACGACGAGTTGTTCTGCGAACT
GCAGATCGAGTTGCTGCGTCACCCGCACGCGGGTGTTCTGATTCAGGGCACTGGCGGCTTGCGCAAGATGCGTTTCGTCG
ATGAGCGACGCAACAAGGGCAGGCGTGGCGGGCTGAGGGTGATTTATTACTGGTGGTCCGGAGGAGCACAGTTCTGGCTC
TTCACCCTGTTCGACAAGGAGCAGATGGACGACCTGCTACCGCAGCAGCGACAACAGCTCAAACAACTGCTGGAACGTGA
AATCGAGGCCAGACGACATGACCAAACGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12064.95 Da        Isoelectric Point: 10.4439

>AT20769 WP_011062198.1 NC_004129:4514794-4515111 [Pseudomonas protegens Pf-5]
MTKRDIFSELVEGIDALTQERQGKITLRSHKVKLEKLPPITAAEVMNIRQQLNLSRSVFAMYLRTNTRTLENWEQGRAIP
NAQATTLIRLVERYPDTVERLASLA

Download         Length: 318 bp

>AT20769 NC_004129:4514794-4515111 [Pseudomonas protegens Pf-5]
ATGACCAAACGTGACATCTTTTCCGAACTGGTAGAAGGCATCGACGCCCTCACCCAGGAACGCCAGGGCAAGATCACCCT
GCGCAGCCATAAAGTGAAGCTCGAAAAGCTGCCGCCCATCACCGCCGCCGAGGTGATGAACATCCGCCAGCAACTCAACC
TCTCGCGCTCGGTATTCGCCATGTACCTGCGCACCAACACCCGCACCCTGGAGAACTGGGAACAGGGCCGCGCCATACCC
AACGCCCAGGCCACGACCTTGATACGCCTGGTGGAACGCTACCCCGATACAGTGGAGCGCCTCGCCTCGCTGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q4K9S3

Antitoxin

Source ID Structure
AlphaFold DB A0A2C9EPZ9

References