Detailed information of TA system TA207446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2307490..2308145
• Replicon: chromosome
• Accession: NZ_CP077678
• Organism: Pseudomonas chlororaphis strain X8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7D6EBU8
• Locus tag: KS461_RS10695
• Protein ID: WP_062823026.1
• Coordinates: 2307490..2307834 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KS461_RS10700
• Protein ID: WP_258257218.1
• Coordinates: 2307831..2308145 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KS461_RS10665 (KS461_10645)	2303143..2304006	+	864	WP_258257211.1	hypothetical protein	-
KS461_RS10670 (KS461_10650)	2304418..2305173	+	756	WP_258257213.1	hypothetical protein	-
KS461_RS10675 (KS461_10655)	2305412..2305912	-	501	WP_258257214.1	helix-turn-helix domain-containing protein	-
KS461_RS10680 (KS461_10660)	2306030..2306188	+	159	WP_258257215.1	Arc family DNA-binding protein	-
KS461_RS10685 (KS461_10665)	2306257..2306565	+	309	WP_258257216.1	DUF3077 domain-containing protein	-
KS461_RS10690 (KS461_10670)	2306565..2307356	+	792	WP_258257217.1	phage antirepressor KilAC domain-containing protein	-
KS461_RS10695 (KS461_10675)	2307490..2307834	+	345	WP_062823026.1	hypothetical protein	Toxin
KS461_RS10700 (KS461_10680)	2307831..2308145	+	315	WP_258257218.1	transcriptional regulator	Antitoxin
KS461_RS10705 (KS461_10685)	2308198..2308623	-	426	WP_102680187.1	hypothetical protein	-
KS461_RS10710 (KS461_10690)	2308723..2308968	+	246	WP_062823023.1	YdaS family helix-turn-helix protein	-
KS461_RS10715 (KS461_10695)	2309093..2309515	+	423	WP_102680186.1	Rha family transcriptional regulator	-
KS461_RS10720 (KS461_10700)	2309615..2310124	+	510	WP_258257220.1	hypothetical protein	-
KS461_RS10725 (KS461_10705)	2310121..2310384	+	264	WP_258257221.1	hypothetical protein	-
KS461_RS10730	2310381..2310602	+	222	WP_258257222.1	hypothetical protein	-
KS461_RS10735 (KS461_10710)	2310932..2311705	+	774	WP_258257223.1	hypothetical protein	-
KS461_RS10740	2311995..2312492	+	498	WP_241190670.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2295031..2328721	33690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13208.96 Da
• Isoelectric Point: 9.6391

>T207446 WP_062823026.1 NZ_CP077678:2307490-2307834 [Pseudomonas chlororaphis]
MKTVFFETTSFTATVGDYLTDDEYRQLQAEMQANPTAGDVMPRTGGFRKLRWHDSRRGKGKRGGLRVIYYWLLDNDQFWM
FSIYDKDEVTKLTSDQEKALKAAIDAELKKRGGK

Nucleotide

• Length: 345 bp

>T207446 NZ_CP103066:2273227-2273343 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12026.03 Da
• Isoelectric Point: 10.0926

>AT207446 WP_258257218.1 NZ_CP077678:2307831-2308145 [Pseudomonas chlororaphis]
MKKRDLFAELMQGVEEMGEHRKGKITLRQYEIEPLPAPEVTAAEIISIREKLHMSQPVFARQIRTSPDTLKNWEQSKSKP
NAQAALLIKLVQRFPDMVERLNTV

Nucleotide

• Length: 315 bp

>AT207446 NZ_CP103066:c2273517-2273338 [Bacillus subtilis]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7D6EBU8