Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3649243..3649970 Replicon chromosome
Accession NZ_CP077660
Organism Enterobacter cloacae complex sp. ECL78

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag KQQ04_RS17490 Protein ID WP_022650547.1
Coordinates 3649243..3649554 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag KQQ04_RS17495 Protein ID WP_022650546.1
Coordinates 3649551..3649970 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQQ04_RS17470 3645514..3646293 - 780 WP_003858848.1 acetolactate decarboxylase -
KQQ04_RS17475 3646399..3647280 + 882 WP_022650550.1 LysR family transcriptional regulator -
KQQ04_RS17480 3647563..3648018 + 456 WP_022650549.1 hypothetical protein -
KQQ04_RS22990 3648131..3648787 - 657 WP_022650548.1 hypothetical protein -
KQQ04_RS17490 3649243..3649554 + 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
KQQ04_RS17495 3649551..3649970 + 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
KQQ04_RS17500 3650294..3650728 + 435 WP_022650545.1 hypothetical protein -
KQQ04_RS17505 3650781..3651671 - 891 WP_022650544.1 oxidoreductase -
KQQ04_RS17510 3651777..3652898 + 1122 Protein_3442 YbdK family carboxylate-amine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T207334 WP_022650547.1 NZ_CP077660:3649243-3649554 [Enterobacter cloacae complex sp. ECL78]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T207334 NZ_CP102961:c825511-825323 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT207334 WP_022650546.1 NZ_CP077660:3649551-3649970 [Enterobacter cloacae complex sp. ECL78]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT207334 NZ_CP102961:c825800-825540 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References