Detailed information of TA system TA207321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4199768..4200498
• Replicon: chromosome
• Accession: NZ_CP077659
• Organism: Enterobacter cloacae complex sp. ECL72

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: KTH07_RS20300
• Protein ID: WP_023302825.1
• Coordinates: 4200184..4200498 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KTH07_RS20295
• Protein ID: WP_023302826.1
• Coordinates: 4199768..4200187 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTH07_RS20270	4195216..4196721	-	1506	WP_017694010.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
KTH07_RS20275	4196718..4197311	-	594	WP_003861054.1	transcriptional regulator UhpA	-
KTH07_RS20280	4197437..4197724	-	288	WP_015570113.1	acetolactate synthase small subunit	-
KTH07_RS20285	4197728..4199416	-	1689	WP_023302827.1	acetolactate synthase large subunit	-
KTH07_RS20290	4199523..4199621	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
KTH07_RS20295	4199768..4200187	-	420	WP_023302826.1	helix-turn-helix domain-containing protein	Antitoxin
KTH07_RS20300	4200184..4200498	-	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
KTH07_RS20305	4201000..4201074	+	75	WP_223237555.1	type I toxin-antitoxin system toxin TisB	-
KTH07_RS20310	4201139..4201585	+	447	WP_003861064.1	GNAT family N-acetyltransferase	-
KTH07_RS20315	4201648..4202481	+	834	WP_015570117.1	DMT family transporter	-
KTH07_RS20320	4202657..4203841	+	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
KTH07_RS20325	4203747..4204832	-	1086	Protein_3973	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T207321 WP_023302825.1 NZ_CP077659:c4200498-4200184 [Enterobacter cloacae complex sp. ECL72]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T207321 NZ_CP102958:c2417656-2417390 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15380.96 Da
• Isoelectric Point: 5.2068

>AT207321 WP_023302826.1 NZ_CP077659:c4200187-4199768 [Enterobacter cloacae complex sp. ECL72]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT207321 NZ_CP102958:c2417913-2417656 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGAATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A837FIL2