Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3152384..3153059 Replicon chromosome
Accession NZ_CP077659
Organism Enterobacter cloacae complex sp. ECL72

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag KTH07_RS15220 Protein ID WP_015571638.1
Coordinates 3152384..3152683 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag KTH07_RS15225 Protein ID WP_015571639.1
Coordinates 3152694..3153059 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTH07_RS15210 3148761..3150953 + 2193 WP_017692801.1 type I secretion system permease/ATPase -
KTH07_RS15215 3150934..3152133 + 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
KTH07_RS15220 3152384..3152683 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KTH07_RS15225 3152694..3153059 + 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
KTH07_RS15230 3153086..3154267 - 1182 WP_032619175.1 PLP-dependent aminotransferase family protein -
KTH07_RS15235 3154288..3155175 - 888 WP_015571641.1 LysR family transcriptional regulator -
KTH07_RS15240 3155273..3155875 + 603 WP_017382759.1 short chain dehydrogenase -
KTH07_RS15245 3155872..3156603 - 732 WP_017382760.1 methyltransferase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T207318 WP_015571638.1 NZ_CP077659:3152384-3152683 [Enterobacter cloacae complex sp. ECL72]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T207318 NZ_CP102958:c2011767-2011405 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT207318 WP_015571639.1 NZ_CP077659:3152694-3153059 [Enterobacter cloacae complex sp. ECL72]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp

>AT207318 NZ_CP102958:c2011934-2011764 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References