Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2455685..2456345 Replicon chromosome
Accession NZ_CP077659
Organism Enterobacter cloacae complex sp. ECL72

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q4X0
Locus tag KTH07_RS11975 Protein ID WP_017383312.1
Coordinates 2455992..2456345 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q4Z7
Locus tag KTH07_RS11970 Protein ID WP_017383313.1
Coordinates 2455685..2455987 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTH07_RS11930 2451367..2451846 + 480 WP_001588075.1 DUF4756 family protein -
KTH07_RS11935 2452005..2452409 + 405 WP_001588074.1 H-NS family nucleoid-associated regulatory protein -
KTH07_RS11940 2452535..2452915 - 381 WP_004151487.1 hypothetical protein -
KTH07_RS11945 2452968..2453258 - 291 WP_002911787.1 hypothetical protein -
KTH07_RS11950 2453337..2453648 - 312 WP_001030749.1 hypothetical protein -
KTH07_RS11955 2453662..2453991 - 330 WP_001588070.1 hypothetical protein -
KTH07_RS11960 2454007..2454225 - 219 WP_002911792.1 AlpA family transcriptional regulator -
KTH07_RS11965 2454373..2455236 - 864 WP_000211446.1 DUF6387 family protein -
KTH07_RS11970 2455685..2455987 - 303 WP_017383313.1 XRE family transcriptional regulator Antitoxin
KTH07_RS11975 2455992..2456345 - 354 WP_017383312.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KTH07_RS11980 2456536..2457486 - 951 WP_032619370.1 HTH-type transcriptional regulator Cbl -
KTH07_RS11985 2457583..2458500 - 918 WP_003859531.1 nitrogen assimilation transcriptional regulator NAC -
KTH07_RS11995 2459033..2459966 - 934 Protein_2360 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(18-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13537.37 Da        Isoelectric Point: 9.9538

>T207317 WP_017383312.1 NZ_CP077659:c2456345-2455992 [Enterobacter cloacae complex sp. ECL72]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLRERGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREYTRWLNTLKERN

Download         Length: 354 bp

>T207317 NZ_CP102958:c1850963-1850811 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGACTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11028.85 Da        Isoelectric Point: 6.4727

>AT207317 WP_017383313.1 NZ_CP077659:c2455987-2455685 [Enterobacter cloacae complex sp. ECL72]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT207317 NZ_CP102958:c1851587-1850988 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q4X0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q4Z7

References