Detailed information of TA system TA207302

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 137659..137913
• Replicon: plasmid p1_ST865
• Accession: NZ_CP077650
• Organism: Escherichia coli strain ST865

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: KTN07_RS23865
• Protein ID: WP_001336447.1
• Coordinates: 137764..137913 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	137659..137720 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTN07_RS23840 (133115)	133115..133861	+	747	WP_194874740.1	conjugal transfer pilus acetylase TraX	-
KTN07_RS23845 (133920)	133920..134780	+	861	WP_000704512.1	alpha/beta hydrolase	-
KTN07_RS23850 (134883)	134883..135440	+	558	WP_194874741.1	fertility inhibition protein FinO	-
KTN07_RS24100 (135592)	135592..135795	+	204	WP_137531148.1	hypothetical protein	-
KTN07_RS23855 (136537)	136537..136998	+	462	WP_194874742.1	thermonuclease family protein	-
KTN07_RS23860 (137101)	137101..137484	+	384	WP_062855964.1	hypothetical protein	-
- (137659)	137659..137720	-	62	NuclAT_0	-	Antitoxin
- (137659)	137659..137720	-	62	NuclAT_0	-	Antitoxin
- (137659)	137659..137720	-	62	NuclAT_0	-	Antitoxin
- (137659)	137659..137720	-	62	NuclAT_0	-	Antitoxin
KTN07_RS23865 (137764)	137764..137913	+	150	WP_001336447.1	Hok/Gef family protein	Toxin
KTN07_RS23870 (138197)	138197..138445	+	249	WP_000083838.1	replication regulatory protein RepA	-
KTN07_RS24105 (138560)	138560..138745	+	186	Protein_156	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeC / faeD / faeE / faeF / faeH / faeI / faeJ	1..138757	138757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T207302 WP_001336447.1 NZ_CP077650:137764-137913 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T207302 NZ_CP102955:c2402808-2402542 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATCGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATTACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT207302 NZ_CP077650:c137720-137659 [Escherichia coli]
TTGAGATACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin