Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3120362..3121161 Replicon chromosome
Accession NZ_CP077649
Organism Escherichia coli strain ST865

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KTN07_RS14925 Protein ID WP_000347267.1
Coordinates 3120362..3120826 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KTN07_RS14930 Protein ID WP_001307405.1
Coordinates 3120826..3121161 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN07_RS14895 (3115363) 3115363..3115797 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KTN07_RS14900 (3115815) 3115815..3116693 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KTN07_RS14905 (3116683) 3116683..3117462 - 780 WP_157702017.1 PTS N-acetylgalactosamine transporter subunit IIC -
KTN07_RS14910 (3117473) 3117473..3117946 - 474 WP_016235805.1 PTS N-acetylgalactosamine transporter subunit IIB -
KTN07_RS14915 (3117969) 3117969..3119249 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KTN07_RS14920 (3119498) 3119498..3120307 + 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
KTN07_RS14925 (3120362) 3120362..3120826 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KTN07_RS14930 (3120826) 3120826..3121161 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KTN07_RS14935 (3121310) 3121310..3122881 - 1572 WP_001273741.1 galactarate dehydratase -
KTN07_RS14940 (3123256) 3123256..3124590 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KTN07_RS14945 (3124606) 3124606..3125376 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3120362..3132035 11673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T207286 WP_000347267.1 NZ_CP077649:c3120826-3120362 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T207286 NZ_CP102953:c816798-816610 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT207286 WP_001307405.1 NZ_CP077649:c3121161-3120826 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT207286 NZ_CP102953:c817087-816827 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References