Detailed information of TA system TA207284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2326450..2327052
• Replicon: chromosome
• Accession: NZ_CP077649
• Organism: Escherichia coli strain ST865

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KTN07_RS11075
• Protein ID: WP_000897305.1
• Coordinates: 2326741..2327052 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KTN07_RS11070
• Protein ID: WP_000356397.1
• Coordinates: 2326450..2326740 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTN07_RS11045 (2322376)	2322376..2323278	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KTN07_RS11050 (2323275)	2323275..2323910	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KTN07_RS11055 (2323907)	2323907..2324836	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KTN07_RS11060 (2325165)	2325165..2325407	-	243	WP_001087409.1	protein YiiF	-
KTN07_RS11065 (2325627)	2325627..2325845	-	219	WP_001315930.1	CopG family transcriptional regulator	-
KTN07_RS11070 (2326450)	2326450..2326740	-	291	WP_000356397.1	NadS family protein	Antitoxin
KTN07_RS11075 (2326741)	2326741..2327052	-	312	WP_000897305.1	hypothetical protein	Toxin
KTN07_RS11080 (2327281)	2327281..2328189	+	909	WP_217609456.1	alpha/beta hydrolase	-
KTN07_RS11085 (2328253)	2328253..2329194	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KTN07_RS11090 (2329239)	2329239..2329676	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KTN07_RS11095 (2329673)	2329673..2330545	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KTN07_RS11100 (2330539)	2330539..2331138	-	600	WP_001295269.1	glucose-1-phosphatase	-
KTN07_RS11105 (2331237)	2331237..2332022	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T207284 WP_000897305.1 NZ_CP077649:c2327052-2326741 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T207284 NZ_CP102952:c2413664-2413398 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT207284 WP_000356397.1 NZ_CP077649:c2326740-2326450 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT207284 NZ_CP102952:c2413915-2413664 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7