Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1511371..1511912 Replicon chromosome
Accession NZ_CP077646
Organism Escherichia coli strain ST866

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KTN06_RS07360 Protein ID WP_000615976.1
Coordinates 1511634..1511912 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KTN06_RS07355 Protein ID WP_000729704.1
Coordinates 1511371..1511631 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTN06_RS07330 (1507154) 1507154..1507939 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
KTN06_RS07335 (1507911) 1507911..1509623 + 1713 Protein_1437 flagellar biosynthesis protein FlhA -
KTN06_RS07340 (1509728) 1509728..1510006 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KTN06_RS07345 (1509999) 1509999..1510355 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
KTN06_RS07350 (1510412) 1510412..1511185 - 774 WP_000543895.1 C40 family peptidase -
KTN06_RS07355 (1511371) 1511371..1511631 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KTN06_RS07360 (1511634) 1511634..1511912 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KTN06_RS07365 (1512068) 1512068..1512808 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KTN06_RS07370 (1512779) 1512779..1513546 - 768 WP_000333380.1 class II glutamine amidotransferase -
KTN06_RS07375 (1513752) 1513752..1514330 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / clpV/tssH / tssA / hcp1/tssD1 1509999..1538106 28107


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T207254 WP_000615976.1 NZ_CP077646:1511634-1511912 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T207254 NZ_CP102947:c1922102-1921728 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT207254 WP_000729704.1 NZ_CP077646:1511371-1511631 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT207254 NZ_CP102947:c1922559-1922191 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References