Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4481223..4481947 Replicon chromosome
Accession NZ_CP077421
Organism Pectobacterium polaris strain QK413-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KSL88_RS20010 Protein ID WP_224072679.1
Coordinates 4481636..4481947 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KSL88_RS20005 Protein ID WP_103861592.1
Coordinates 4481223..4481639 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KSL88_RS19985 (KSL88_19940) 4476741..4478093 + 1353 WP_224072678.1 pyridoxal-dependent decarboxylase -
KSL88_RS19990 (KSL88_19945) 4478188..4478646 - 459 WP_075277791.1 hypothetical protein -
KSL88_RS19995 (KSL88_19950) 4479916..4480065 - 150 Protein_3915 type I-E CRISPR-associated endoribonuclease Cas2e -
KSL88_RS20000 (KSL88_19955) 4480492..4480995 + 504 WP_095701369.1 hypothetical protein -
KSL88_RS20005 (KSL88_19960) 4481223..4481639 - 417 WP_103861592.1 helix-turn-helix domain-containing protein Antitoxin
KSL88_RS20010 (KSL88_19965) 4481636..4481947 - 312 WP_224072679.1 type II toxin-antitoxin system HigB family toxin Toxin
KSL88_RS20015 (KSL88_19970) 4483473..4483766 - 294 WP_005973730.1 type I-E CRISPR-associated endoribonuclease Cas2e -
KSL88_RS20020 (KSL88_19975) 4483767..4484684 - 918 WP_224072680.1 type I-E CRISPR-associated endonuclease Cas1e -
KSL88_RS20025 (KSL88_19980) 4484712..4485359 - 648 WP_224073902.1 type I-E CRISPR-associated protein Cas6/Cse3/CasE -
KSL88_RS20030 (KSL88_19985) 4485359..4486093 - 735 WP_107332211.1 type I-E CRISPR-associated protein Cas5/CasD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4480492..4490722 10230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12423.22 Da        Isoelectric Point: 9.6392

>T207194 WP_224072679.1 NZ_CP077421:c4481947-4481636 [Pectobacterium polaris]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGTI
FIKHITSHAEYDRLTEYYRRNKE

Download         Length: 312 bp

>T207194 NZ_CP102932:c2796783-2796680 [Enterobacter sp. Crenshaw]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15405.70 Da        Isoelectric Point: 4.8037

>AT207194 WP_103861592.1 NZ_CP077421:c4481639-4481223 [Pectobacterium polaris]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Download         Length: 417 bp

>AT207194 NZ_CP102932:2796674-2796818 [Enterobacter sp. Crenshaw]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References