Detailed information of TA system TA207194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4481223..4481947
• Replicon: chromosome
• Accession: NZ_CP077421
• Organism: Pectobacterium polaris strain QK413-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KSL88_RS20010
• Protein ID: WP_224072679.1
• Coordinates: 4481636..4481947 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KSL88_RS20005
• Protein ID: WP_103861592.1
• Coordinates: 4481223..4481639 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSL88_RS19985 (KSL88_19940)	4476741..4478093	+	1353	WP_224072678.1	pyridoxal-dependent decarboxylase	-
KSL88_RS19990 (KSL88_19945)	4478188..4478646	-	459	WP_075277791.1	hypothetical protein	-
KSL88_RS19995 (KSL88_19950)	4479916..4480065	-	150	Protein_3915	type I-E CRISPR-associated endoribonuclease Cas2e	-
KSL88_RS20000 (KSL88_19955)	4480492..4480995	+	504	WP_095701369.1	hypothetical protein	-
KSL88_RS20005 (KSL88_19960)	4481223..4481639	-	417	WP_103861592.1	helix-turn-helix domain-containing protein	Antitoxin
KSL88_RS20010 (KSL88_19965)	4481636..4481947	-	312	WP_224072679.1	type II toxin-antitoxin system HigB family toxin	Toxin
KSL88_RS20015 (KSL88_19970)	4483473..4483766	-	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
KSL88_RS20020 (KSL88_19975)	4483767..4484684	-	918	WP_224072680.1	type I-E CRISPR-associated endonuclease Cas1e	-
KSL88_RS20025 (KSL88_19980)	4484712..4485359	-	648	WP_224073902.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
KSL88_RS20030 (KSL88_19985)	4485359..4486093	-	735	WP_107332211.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4480492..4490722	10230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12423.22 Da
• Isoelectric Point: 9.6392

>T207194 WP_224072679.1 NZ_CP077421:c4481947-4481636 [Pectobacterium polaris]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGTI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T207194 NZ_CP102932:c2796783-2796680 [Enterobacter sp. Crenshaw]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15405.70 Da
• Isoelectric Point: 4.8037

>AT207194 WP_103861592.1 NZ_CP077421:c4481639-4481223 [Pectobacterium polaris]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT207194 NZ_CP102932:2796674-2796818 [Enterobacter sp. Crenshaw]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT