Detailed information of TA system TA207175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2314856..2315516
• Replicon: chromosome
• Accession: NZ_CP077411
• Organism: Enterobacter asburiae strain FDAARGOS 1432

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6L62_RS11170
• Protein ID: WP_058841455.1
• Coordinates: 2315163..2315516 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: I6L62_RS11165
• Protein ID: WP_029741460.1
• Coordinates: 2314856..2315158 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L62_RS11125 (I6L62_11125)	2309984..2310370	-	387	WP_048234408.1	hypothetical protein	-
I6L62_RS11130 (I6L62_11130)	2310385..2310696	-	312	WP_001515481.1	hypothetical protein	-
I6L62_RS11135 (I6L62_11135)	2310726..2310941	-	216	WP_000250489.1	AlpA family transcriptional regulator	-
I6L62_RS11140 (I6L62_11140)	2311128..2311991	-	864	WP_032278482.1	DUF6387 family protein	-
I6L62_RS11145 (I6L62_11145)	2312448..2312780	-	333	WP_032658722.1	type II toxin-antitoxin system PemK/MazF family toxin	-
I6L62_RS11150 (I6L62_11150)	2312781..2313038	-	258	WP_033145928.1	antitoxin	-
I6L62_RS11155 (I6L62_11155)	2313329..2313718	-	390	WP_059445031.1	RidA family protein	-
I6L62_RS11160 (I6L62_11160)	2313860..2314780	+	921	WP_217073177.1	LysR family transcriptional regulator	-
I6L62_RS11165 (I6L62_11165)	2314856..2315158	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
I6L62_RS11170 (I6L62_11170)	2315163..2315516	-	354	WP_058841455.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L62_RS11175 (I6L62_11175)	2315694..2316587	-	894	WP_217073178.1	LysR substrate-binding domain-containing protein	-
I6L62_RS11180 (I6L62_11180)	2316758..2317366	+	609	WP_217073179.1	glutathione S-transferase family protein	-
I6L62_RS11185 (I6L62_11185)	2317410..2318360	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
I6L62_RS11190 (I6L62_11190)	2318457..2319374	-	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2310726..2323579	12853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13522.32 Da
• Isoelectric Point: 7.1678

>T207175 WP_058841455.1 NZ_CP077411:c2315516-2315163 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
MLCAGNKVGNERRFYDEMLLVADQEFTHWLDTLKERE

Nucleotide

• Length: 354 bp

>T207175 NZ_CP102928:c784179-783889 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT207175 WP_029741460.1 NZ_CP077411:c2315158-2314856 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT207175 NZ_CP102928:c784417-784169 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79