Detailed information of TA system TA207095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 5388..5973
• Replicon: plasmid unnamed4
• Accession: NZ_CP077373
• Organism: Acinetobacter lwoffii strain FDAARGOS 1394

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6L24_RS16275
• Protein ID: WP_216986677.1
• Coordinates: 5653..5973 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L24_RS16270
• Protein ID: WP_216986676.1
• Coordinates: 5388..5660 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L24_RS16245 (I6L24_16250)	837..1373	+	537	WP_216986675.1	plasmid replication DNA-binding protein	-
I6L24_RS16250 (I6L24_16255)	1656..3110	-	1455	WP_131275007.1	SulP family inorganic anion transporter	-
I6L24_RS16255 (I6L24_16260)	3358..3567	-	210	WP_010116191.1	hypothetical protein	-
I6L24_RS16260 (I6L24_16265)	3950..4330	+	381	WP_005225409.1	fluoride efflux transporter CrcB	-
I6L24_RS16265 (I6L24_16270)	4357..4881	+	525	WP_131275023.1	inorganic diphosphatase	-
I6L24_RS16270 (I6L24_16275)	5388..5660	-	273	WP_216986676.1	NadS family protein	Antitoxin
I6L24_RS16275 (I6L24_16280)	5653..5973	-	321	WP_216986677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L24_RS16280 (I6L24_16285)	6118..6717	+	600	WP_216986678.1	hypothetical protein	-
I6L24_RS16285 (I6L24_16290)	7163..7354	-	192	WP_004282089.1	YqaE/Pmp3 family membrane protein	-
I6L24_RS16290 (I6L24_16295)	7384..7665	-	282	WP_216986680.1	mobilization protein	-
I6L24_RS16295 (I6L24_16300)	7875..9311	+	1437	WP_216986679.1	MobQ family relaxase	-
I6L24_RS16300 (I6L24_16305)	9837..10760	+	924	WP_004282204.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19982	19982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12208.07 Da
• Isoelectric Point: 9.3987

>T207095 WP_216986677.1 NZ_CP077373:c5973-5653 [Acinetobacter lwoffii]
MLFIETSIFTKQIKELVSDKEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T207095 NZ_CP102883:351963-352310 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10081.53 Da
• Isoelectric Point: 7.2045

>AT207095 WP_216986676.1 NZ_CP077373:c5660-5388 [Acinetobacter lwoffii]
MDNSLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT207095 NZ_CP102883:352310-352609 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA