Detailed information of TA system TA207089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 233052..233873
• Replicon: plasmid unnamed2
• Accession: NZ_CP077368
• Organism: Pantoea agglomerans strain FDAARGOS 1447

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A379LR32
• Locus tag: I6L77_RS20440
• Protein ID: WP_045140153.1
• Coordinates: 233403..233873 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A8X8DUC8
• Locus tag: I6L77_RS20435
• Protein ID: WP_033773852.1
• Coordinates: 233052..233396 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L77_RS20415 (I6L77_20410)	229054..230163	+	1110	WP_010247360.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-
I6L77_RS20420 (I6L77_20415)	230207..231508	+	1302	WP_033760237.1	maltoporin	-
I6L77_RS20425 (I6L77_20420)	231611..232549	+	939	WP_062759189.1	maltose operon protein MalM	-
I6L77_RS20430 (I6L77_20425)	232786..232953	+	168	WP_235330831.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
I6L77_RS20435 (I6L77_20430)	233052..233396	+	345	WP_033773852.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
I6L77_RS20440 (I6L77_20435)	233403..233873	+	471	WP_045140153.1	type II toxin-antitoxin system YhaV family toxin	Toxin
I6L77_RS20445 (I6L77_20440)	234062..236137	-	2076	WP_062759191.1	4-alpha-glucanotransferase	-
I6L77_RS20450 (I6L77_20445)	236146..238548	-	2403	WP_062759193.1	maltodextrin phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hcp/tssD / iroN	1..511735	511735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 18307.94 Da
• Isoelectric Point: 8.9277

>T207089 WP_045140153.1 NZ_CP077368:233403-233873 [Pantoea agglomerans]
MEDSTVINGWHVYVHPCFESQLIALTNEVELLRAKYPESYQRKATTKLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKRAYGSMRDAYKVFSGMLHNGYPPDDWALLLKQSQDWAKINS

Nucleotide

• Length: 471 bp

>T207089 NZ_CP102878:46685-46972 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12285.96 Da
• Isoelectric Point: 4.3278

>AT207089 WP_033773852.1 NZ_CP077368:233052-233396 [Pantoea agglomerans]
MGTLTHANISLRASSKLTARSQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVTDDDPVMASFLSFLAADIRNT
PANIKTLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT207089 NZ_CP102878:46446-46685 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A379LR32