Detailed information of TA system TA207080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2280039..2280769
• Replicon: chromosome
• Accession: NZ_CP077366
• Organism: Pantoea agglomerans strain FDAARGOS 1447

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A356RQP5
• Locus tag: I6L77_RS10365
• Protein ID: WP_010670815.1
• Coordinates: 2280452..2280769 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L77_RS10360
• Protein ID: WP_010258577.1
• Coordinates: 2280039..2280371 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L77_RS10340 (I6L77_10340)	2275417..2276058	+	642	WP_003849160.1	protein phosphatase CheZ	-
I6L77_RS10345 (I6L77_10345)	2276247..2277398	+	1152	WP_010258565.1	flagellar biosynthesis protein FlhB	-
I6L77_RS10350 (I6L77_10350)	2277391..2279484	+	2094	WP_010258569.1	flagellar biosynthesis protein FlhA	-
I6L77_RS10355 (I6L77_10355)	2279484..2279876	+	393	WP_062757847.1	flagellar protein FlhE	-
I6L77_RS10360 (I6L77_10360)	2280039..2280371	-	333	WP_010258577.1	HigA family addiction module antitoxin	Antitoxin
I6L77_RS10365 (I6L77_10365)	2280452..2280769	-	318	WP_010670815.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L77_RS10370 (I6L77_10370)	2280844..2281842	-	999	WP_062757846.1	aldo/keto reductase	-
I6L77_RS10375 (I6L77_10375)	2282055..2282990	-	936	WP_062757844.1	LysR family transcriptional regulator	-
I6L77_RS10380 (I6L77_10380)	2283078..2284808	-	1731	WP_062757842.1	arginine--tRNA ligase	-
I6L77_RS21895	2285019..2285141	-	123	WP_009089698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (8-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12284.10 Da
• Isoelectric Point: 10.1171

>T207080 WP_010670815.1 NZ_CP077366:c2280769-2280452 [Pantoea agglomerans]
VATLRNIESFRDDWLKDYFLYGKFSKRIPSSLSSALARKLDIINAAISYKDLKSPPGNRYEELNPPLKGYASIRVNEQYR
LIFKWIEGKAVDLYLDAHSYKTHKR

Nucleotide

• Length: 318 bp

>T207080 NZ_CP077366:c2280769-2280452 [Pantoea agglomerans]
GTGGCGACGTTAAGGAATATTGAAAGTTTCAGGGATGACTGGCTTAAGGATTATTTTTTATATGGCAAATTTTCCAAAAG
AATACCGTCAAGCCTCTCATCAGCGCTGGCGCGAAAGCTCGACATCATCAATGCGGCGATTAGCTATAAGGATTTAAAGT
CGCCGCCAGGCAATCGTTATGAGGAACTAAACCCGCCTCTCAAGGGATATGCTTCAATCAGGGTCAATGAGCAATATCGG
CTGATCTTTAAATGGATAGAAGGTAAGGCCGTTGACCTTTATCTGGATGCTCATAGCTATAAAACGCACAAGCGATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12615.48 Da
• Isoelectric Point: 5.1473

>AT207080 WP_010258577.1 NZ_CP077366:c2280371-2280039 [Pantoea agglomerans]
MNQATRKPTTVGDVLLYEYLEPTGLKILDLAEMLKVHRNTVSALVNNNRKLTPDMAFRLAAAFETSVEFWLNLQNSVDIW
EVMHDARAQEEISRVTPLKDFLVQKENQLA

Nucleotide

• Length: 333 bp

>AT207080 NZ_CP077366:c2280371-2280039 [Pantoea agglomerans]
ATGAACCAGGCGACCCGCAAACCGACCACTGTCGGTGACGTATTACTTTACGAATATCTGGAGCCAACCGGCCTGAAGAT
TCTCGACTTAGCGGAGATGCTAAAAGTGCACCGCAACACCGTCAGCGCCCTGGTCAACAATAACCGGAAGCTGACGCCGG
ATATGGCTTTCAGGCTGGCTGCAGCCTTCGAAACCAGCGTTGAGTTCTGGCTTAATCTGCAAAACAGTGTCGATATCTGG
GAAGTGATGCATGATGCCAGGGCACAGGAGGAGATCAGTCGCGTAACACCGTTGAAGGATTTTCTGGTGCAGAAAGAGAA
TCAGCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A356RQP5