Detailed information of TA system TA207077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 255777..256429
• Replicon: chromosome
• Accession: NZ_CP077366
• Organism: Pantoea agglomerans strain FDAARGOS 1447

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8X8DRT2
• Locus tag: I6L77_RS01160
• Protein ID: WP_010672045.1
• Coordinates: 255777..256130 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A379AMS3
• Locus tag: I6L77_RS01165
• Protein ID: WP_062759345.1
• Coordinates: 256130..256429 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L77_RS01145 (I6L77_01145)	251503..253287	-	1785	WP_010252112.1	GMC family oxidoreductase	-
I6L77_RS01150 (I6L77_01150)	253290..254024	-	735	WP_033787533.1	gluconate 2-dehydrogenase subunit 3 family protein	-
I6L77_RS01155 (I6L77_01155)	254301..255542	+	1242	WP_010672044.1	peptide antibiotic transporter SbmA	-
I6L77_RS01160 (I6L77_01160)	255777..256130	+	354	WP_010672045.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L77_RS01165 (I6L77_01165)	256130..256429	+	300	WP_062759345.1	XRE family transcriptional regulator	Antitoxin
I6L77_RS01170 (I6L77_01170)	256551..257603	-	1053	WP_061901847.1	YncE family protein	-
I6L77_RS01175 (I6L77_01175)	257806..258015	-	210	WP_062759344.1	DUF1471 domain-containing protein	-
I6L77_RS01180 (I6L77_01180)	258228..259415	-	1188	WP_062759343.1	MFS transporter	-
I6L77_RS01185 (I6L77_01185)	259520..260485	+	966	WP_062759342.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13660.67 Da
• Isoelectric Point: 7.9792

>T207077 WP_010672045.1 NZ_CP077366:255777-256130 [Pantoea agglomerans]
MWTVLLSPRFESWLSEQEEALQEKMLADLGKLKVYGPDLPRPYADTLKGSQYRNMKELRVQFSGKPIRAFYAFDPVRQAI
VLCAGDKSHDKRFYIRMIRIADDEFSAWLAEQEKGNN

Nucleotide

• Length: 354 bp

>T207077 NZ_CP102877:351963-352310 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11131.02 Da
• Isoelectric Point: 6.3781

>AT207077 WP_062759345.1 NZ_CP077366:256130-256429 [Pantoea agglomerans]
MRTLDDVIADFSPETQARIKKMSDEMILEVGLQMIREELQLSQKTLAEAMGVRQPAVAQIEQRGNEIKVATLKRYVEAMG
GKLSLDVELPTGKRVAFRI

Nucleotide

• Length: 300 bp

>AT207077 NZ_CP102877:352310-352609 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A379AMS3