Detailed information of TA system TA207072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 115995..116646
• Replicon: plasmid unnamed2
• Accession: NZ_CP077338
• Organism: Acinetobacter lwoffii strain FDAARGOS 1393

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9FYW4
• Locus tag: I6L23_RS16010
• Protein ID: WP_000269911.1
• Coordinates: 116290..116646 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L23_RS16005
• Protein ID: WP_001140620.1
• Coordinates: 115995..116297 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L23_RS15980 (I6L23_15980)	112137..112397	+	261	WP_004665962.1	FeoA family protein	-
I6L23_RS15985 (I6L23_15985)	112387..114216	+	1830	WP_130908662.1	ferrous iron transporter B	-
I6L23_RS15990 (I6L23_15990)	114305..115294	-	990	WP_130908661.1	arsenic resistance protein	-
I6L23_RS15995 (I6L23_15995)	115305..115739	-	435	Protein_126	recombinase family protein	-
I6L23_RS16000 (I6L23_16000)	115792..115953	+	162	Protein_127	IS6 family transposase	-
I6L23_RS16005 (I6L23_16005)	115995..116297	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
I6L23_RS16010 (I6L23_16010)	116290..116646	-	357	WP_000269911.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L23_RS16015 (I6L23_16015)	117426..117755	+	330	WP_004733205.1	four-helix bundle copper-binding protein	-
I6L23_RS16020 (I6L23_16020)	117885..118178	-	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6L23_RS16025 (I6L23_16025)	118168..118413	-	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
I6L23_RS16030 (I6L23_16030)	118593..119537	-	945	WP_004668309.1	copper resistance protein B	-
I6L23_RS16035 (I6L23_16035)	119524..119862	-	339	WP_004897649.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..221423	221423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13600.60 Da
• Isoelectric Point: 7.1651

>T207072 WP_000269911.1 NZ_CP077338:c116646-116290 [Acinetobacter lwoffii]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T207072 NZ_CP102871:85158-86483 [Klebsiella pneumoniae]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT207072 WP_001140620.1 NZ_CP077338:c116297-115995 [Acinetobacter lwoffii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT207072 NZ_CP102871:84860-85153 [Klebsiella pneumoniae]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A6A1RIA3