Detailed information of TA system TA207071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 97438..98089
• Replicon: plasmid unnamed2
• Accession: NZ_CP077338
• Organism: Acinetobacter lwoffii strain FDAARGOS 1393

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: I6L23_RS15905
• Protein ID: WP_004668307.1
• Coordinates: 97733..98089 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L23_RS15900
• Protein ID: WP_001140620.1
• Coordinates: 97438..97740 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L23_RS15875 (I6L23_15875)	93461..94702	+	1242	WP_004644827.1	group II intron reverse transcriptase/maturase	-
I6L23_RS15880 (I6L23_15880)	94782..95252	+	471	Protein_103	IS3 family transposase	-
I6L23_RS15885 (I6L23_15885)	95379..96659	-	1281	WP_004897603.1	cation transporter	-
I6L23_RS15890 (I6L23_15890)	96749..97141	+	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
I6L23_RS15895 (I6L23_15895)	97235..97396	+	162	Protein_106	IS6 family transposase	-
I6L23_RS15900 (I6L23_15900)	97438..97740	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
I6L23_RS15905 (I6L23_15905)	97733..98089	-	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L23_RS15910 (I6L23_15910)	98727..99704	+	978	WP_004761028.1	TerC family protein	-
I6L23_RS15915 (I6L23_15915)	99975..100184	-	210	Protein_110	recombinase family protein	-
I6L23_RS15920 (I6L23_15920)	100236..100940	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
I6L23_RS15925 (I6L23_15925)	100992..101147	+	156	Protein_112	IS5/IS1182 family transposase	-
I6L23_RS15930 (I6L23_15930)	101345..101524	-	180	Protein_113	hypothetical protein	-
I6L23_RS15935 (I6L23_15935)	101728..102627	-	900	WP_130908664.1	cation diffusion facilitator family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..221423	221423
	-	inside	IScluster/Tn	-	-	91544..100940	9396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T207071 WP_004668307.1 NZ_CP077338:c98089-97733 [Acinetobacter lwoffii]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T207071 NZ_CP102870:c139134-138709 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT207071 WP_001140620.1 NZ_CP077338:c97740-97438 [Acinetobacter lwoffii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT207071 NZ_CP102870:c139361-139131 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: N9NM16