Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 245144..245746 Replicon plasmid unnamed1
Accession NZ_CP077309
Organism Enterobacter hormaechei strain FDAARGOS 1433

Toxin (Protein)


Gene name higB Uniprot ID F5RRB8
Locus tag I6L63_RS23940 Protein ID WP_006808714.1
Coordinates 245435..245746 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID F5RRB7
Locus tag I6L63_RS21340 Protein ID WP_006808713.1
Coordinates 245144..245434 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L63_RS21325 (I6L63_21325) 242642..243544 + 903 WP_003861960.1 formate dehydrogenase subunit beta -
I6L63_RS21330 (I6L63_21330) 243541..244176 + 636 WP_006808711.1 formate dehydrogenase cytochrome b556 subunit -
I6L63_RS21335 (I6L63_21335) 244173..245102 + 930 WP_006808712.1 formate dehydrogenase accessory protein FdhE -
I6L63_RS21340 (I6L63_21340) 245144..245434 - 291 WP_006808713.1 NadS family protein Antitoxin
I6L63_RS23940 245435..245746 - 312 WP_006808714.1 hypothetical protein Toxin
I6L63_RS21350 (I6L63_21350) 245895..246836 - 942 WP_032673470.1 fatty acid biosynthesis protein FabY -
I6L63_RS21355 (I6L63_21355) 246881..247318 - 438 WP_006808716.1 D-aminoacyl-tRNA deacylase -
I6L63_RS21360 (I6L63_21360) 247315..248196 - 882 WP_003861949.1 virulence factor BrkB family protein -
I6L63_RS21365 (I6L63_21365) 248190..248789 - 600 WP_000965688.1 glucose-1-phosphatase -
I6L63_RS21370 (I6L63_21370) 248908..249708 - 801 WP_032673471.1 DeoR/GlpR family DNA-binding transcription regulator -
I6L63_RS21375 (I6L63_21375) 249743..250639 - 897 WP_032673472.1 sugar kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - wza / rfaD / lpfA / lpfB / lpfC / lpfD / lpfE 1..606597 606597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12275.30 Da        Isoelectric Point: 9.9930

>T207035 WP_006808714.1 NZ_CP077309:c245746-245435 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPEYGDLIQNTGGLRKIRWLAGGKGKRGGVRVIYFHRTREFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Download         Length: 312 bp

>T207035 NZ_CP102853:2017427-2017530 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10721.23 Da        Isoelectric Point: 10.5888

>AT207035 WP_006808713.1 NZ_CP077309:c245434-245144 [Enterobacter hormaechei]
MEKALFERLTQSIAQMNEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVITALSQ

Download         Length: 291 bp

>AT207035 NZ_CP102853:c2017568-2017297 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F5RRB8

Antitoxin

Source ID Structure
AlphaFold DB A0A7T1QN46

References