Detailed information of TA system TA207032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3149518..3150179
• Replicon: chromosome
• Accession: NZ_CP077308
• Organism: Enterobacter hormaechei strain FDAARGOS 1433

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6L63_RS15205
• Protein ID: WP_226865158.1
• Coordinates: 3149518..3149763 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: F5RUL2
• Locus tag: I6L63_RS15210
• Protein ID: WP_006809705.1
• Coordinates: 3149760..3150179 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L63_RS15185 (I6L63_15185)	3145827..3146606	-	780	WP_003858848.1	acetolactate decarboxylase	-
I6L63_RS15190 (I6L63_15190)	3146712..3147593	+	882	WP_006809701.1	LysR family transcriptional regulator	-
I6L63_RS15195 (I6L63_15195)	3147880..3148335	+	456	WP_006809702.1	hypothetical protein	-
I6L63_RS15200 (I6L63_15200)	3148547..3149296	-	750	WP_032673600.1	hypothetical protein	-
I6L63_RS15205 (I6L63_15205)	3149518..3149763	+	246	WP_226865158.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6L63_RS15210 (I6L63_15210)	3149760..3150179	+	420	WP_006809705.1	helix-turn-helix domain-containing protein	Antitoxin
I6L63_RS15215 (I6L63_15215)	3150232..3150384	-	153	Protein_2986	ketopantoate reductase C-terminal domain-containing protein	-
I6L63_RS15220 (I6L63_15220)	3150380..3150603	+	224	Protein_2987	glutamate-cysteine ligase family protein	-
I6L63_RS15225 (I6L63_15225)	3150947..3151438	+	492	WP_006809707.1	Hcp family type VI secretion system effector	-
I6L63_RS15230 (I6L63_15230)	3151449..3151805	+	357	WP_006809708.1	DUF2778 domain-containing protein	-
I6L63_RS15235 (I6L63_15235)	3151802..3152236	+	435	WP_006809709.1	hypothetical protein	-
I6L63_RS15240 (I6L63_15240)	3152290..3153180	-	891	WP_006809710.1	oxidoreductase	-
I6L63_RS15245 (I6L63_15245)	3153286..3154407	+	1122	WP_006809711.1	YbdK family carboxylate-amine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (2-71)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 10088.63 Da
• Isoelectric Point: 9.8340

>T207032 WP_226865158.1 NZ_CP077308:3149518-3149763 [Enterobacter hormaechei]
MDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIEVGGGHLRVMFFADFERGKIFIKHISTHAEYDKLTDYYRRTK
E

Nucleotide

• Length: 246 bp

>T207032 NZ_CP102853:1333406-1333804 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15447.51 Da
• Isoelectric Point: 4.5951

>AT207032 WP_006809705.1 NZ_CP077308:3149760-3150179 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSTPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT207032 NZ_CP102853:1333179-1333406 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7T1QZI6