Detailed information of TA system TA207009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4812302..4813062
• Replicon: chromosome
• Accession: NZ_CP077300
• Organism: Citrobacter braakii strain FDAARGOS 1422

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: I6L52_RS22665
• Protein ID: WP_019077936.1
• Coordinates: 4812302..4812613 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L52_RS22670
• Protein ID: WP_141876133.1
• Coordinates: 4812610..4813062 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L52_RS22635 (4807971)	4807971..4808873	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
I6L52_RS22640 (4808870)	4808870..4809505	+	636	WP_115191165.1	formate dehydrogenase cytochrome b556 subunit	-
I6L52_RS22645 (4809502)	4809502..4810431	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
I6L52_RS22650 (4810468)	4810468..4810842	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	-
I6L52_RS22655 (4810842)	4810842..4811084	-	243	WP_016155181.1	CopG family transcriptional regulator	-
I6L52_RS22660 (4811289)	4811289..4812217	+	929	Protein_4424	alpha/beta hydrolase	-
I6L52_RS22665 (4812302)	4812302..4812613	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6L52_RS22670 (4812610)	4812610..4813062	+	453	WP_141876133.1	helix-turn-helix domain-containing protein	Antitoxin
I6L52_RS22675 (4813080)	4813080..4814021	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
I6L52_RS22680 (4814066)	4814066..4814503	-	438	WP_003028671.1	D-aminoacyl-tRNA deacylase	-
I6L52_RS22685 (4814500)	4814500..4815372	-	873	WP_019077938.1	virulence factor BrkB family protein	-
I6L52_RS22690 (4815366)	4815366..4815965	-	600	WP_016157873.1	glucose-1-phosphatase	-
I6L52_RS22695 (4816056)	4816056..4816946	-	891	WP_016157872.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T207009 WP_019077936.1 NZ_CP077300:4812302-4812613 [Citrobacter braakii]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T207009 NZ_CP102841:c3187321-3187145 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGCATCAAATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16998.39 Da
• Isoelectric Point: 5.9259

>AT207009 WP_141876133.1 NZ_CP077300:4812610-4813062 [Citrobacter braakii]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT207009 NZ_CP102841:c3187097-3186690 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACAGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCTTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Structures

Toxin

• AlphaFold DB: A0A7L6U900