Detailed information of TA system TA206948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2651530..2652260
• Replicon: chromosome
• Accession: NZ_CP077290
• Organism: Enterobacter cancerogenus strain FDAARGOS 1428

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6L58_RS12510
• Protein ID: WP_058609917.1
• Coordinates: 2651946..2652260 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L58_RS12505
• Protein ID: WP_140418816.1
• Coordinates: 2651530..2651949 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L58_RS12480 (I6L58_12480)	2647004..2648509	-	1506	WP_006177622.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
I6L58_RS12485 (I6L58_12485)	2648506..2649099	-	594	WP_006177621.1	transcriptional regulator UhpA	-
I6L58_RS12490 (I6L58_12490)	2649199..2649486	-	288	WP_006177620.1	acetolactate synthase small subunit	-
I6L58_RS12495 (I6L58_12495)	2649490..2651178	-	1689	WP_088208942.1	acetolactate synthase large subunit	-
I6L58_RS12500 (I6L58_12500)	2651281..2651379	-	99	WP_088208941.1	ilvB operon leader peptide IvbL	-
I6L58_RS12505 (I6L58_12505)	2651530..2651949	-	420	WP_140418816.1	helix-turn-helix domain-containing protein	Antitoxin
I6L58_RS12510 (I6L58_12510)	2651946..2652260	-	315	WP_058609917.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6L58_RS12515 (I6L58_12515)	2652749..2652838	+	90	WP_071599731.1	type I toxin-antitoxin system toxin TisB	-
I6L58_RS12520 (I6L58_12520)	2652903..2653349	+	447	WP_006177617.1	GNAT family N-acetyltransferase	-
I6L58_RS12525 (I6L58_12525)	2653412..2654245	+	834	WP_088208940.1	EamA family transporter	-
I6L58_RS12530 (I6L58_12530)	2654421..2655605	+	1185	WP_088209051.1	multidrug efflux MFS transporter EmrD	-
I6L58_RS12535 (I6L58_12535)	2655508..2656596	-	1089	WP_088208939.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp/tssD	2642980..2652838	9858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12426.57 Da
• Isoelectric Point: 10.2219

>T206948 WP_058609917.1 NZ_CP077290:c2652260-2651946 [Enterobacter cancerogenus]
MHLISMKAIFDAVTLFPQHKQELLFLGRIIEKSNCPTPDALKKLFPTLDNFKYLDKHYVLDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T206948 NZ_CP102819:3861-4148 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15487.01 Da
• Isoelectric Point: 4.9192

>AT206948 WP_140418816.1 NZ_CP077290:c2651949-2651530 [Enterobacter cancerogenus]
MMIVADAMKATHALVAAVPLLGEHPNEQDYNDALELVEYLLMNEPGSPLLDIVCARIRHYEANQPEIVALRQEMESVPVG
IAVLRTLMDQYKLTLSDFQDEIGSKSMVSRVLNGQRQLTLTHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT206948 NZ_CP102819:4135-4449 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA