Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4441320..4441995 Replicon chromosome
Accession NZ_CP077264
Organism Enterobacter hormaechei strain FDAARGOS 1434

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag I6L64_RS21230 Protein ID WP_015571638.1
Coordinates 4441696..4441995 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag I6L64_RS21225 Protein ID WP_015571639.1
Coordinates 4441320..4441685 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L64_RS21205 (I6L64_21205) 4437776..4438507 + 732 WP_017382760.1 methyltransferase domain-containing protein -
I6L64_RS21210 (I6L64_21210) 4438504..4439106 - 603 WP_069595987.1 short chain dehydrogenase -
I6L64_RS21215 (I6L64_21215) 4439204..4440091 + 888 WP_015571641.1 LysR family transcriptional regulator -
I6L64_RS21220 (I6L64_21220) 4440112..4441293 + 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
I6L64_RS21225 (I6L64_21225) 4441320..4441685 - 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
I6L64_RS21230 (I6L64_21230) 4441696..4441995 - 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6L64_RS21235 (I6L64_21235) 4442246..4443445 - 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
I6L64_RS21240 (I6L64_21240) 4443426..4445618 - 2193 WP_017692801.1 type I secretion system permease/ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T206908 WP_015571638.1 NZ_CP077264:c4441995-4441696 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T206908 NZ_CP102749:4359978-4360232 [Pectobacterium parvum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATAGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTGGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT206908 WP_015571639.1 NZ_CP077264:c4441685-4441320 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp

>AT206908 NZ_CP102749:4359730-4359981 [Pectobacterium parvum]
ATGCGTACAATTAGCTACAGCGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGATCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTGATGTCCCTGGAAGAGTATAACTCTCTAGAAGAAACCGCGTATT
TACTGCGTTCACCAGCCAATGCAAAAAGACTGATGAATTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References