Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2091905..2092632 Replicon chromosome
Accession NZ_CP077264
Organism Enterobacter hormaechei strain FDAARGOS 1434

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q486
Locus tag I6L64_RS10025 Protein ID WP_033486526.1
Coordinates 2091905..2092216 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q1H0
Locus tag I6L64_RS10030 Protein ID WP_045349893.1
Coordinates 2092213..2092632 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L64_RS10010 (I6L64_10010) 2087174..2088484 - 1311 WP_137984239.1 hypothetical protein -
I6L64_RS10015 (I6L64_10015) 2088436..2088864 - 429 WP_137984238.1 hypothetical protein -
I6L64_RS10020 (I6L64_10020) 2089363..2091483 + 2121 WP_033486523.1 hypothetical protein -
I6L64_RS10025 (I6L64_10025) 2091905..2092216 + 312 WP_033486526.1 type II toxin-antitoxin system HigB family toxin Toxin
I6L64_RS10030 (I6L64_10030) 2092213..2092632 + 420 WP_045349893.1 helix-turn-helix domain-containing protein Antitoxin
I6L64_RS10035 (I6L64_10035) 2092685..2093575 - 891 WP_069595705.1 oxidoreductase -
I6L64_RS10040 (I6L64_10040) 2093681..2094802 + 1122 WP_015571201.1 YbdK family carboxylate-amine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12506.30 Da        Isoelectric Point: 9.9435

>T206902 WP_033486526.1 NZ_CP077264:2091905-2092216 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Download         Length: 312 bp

>T206902 NZ_CP102749:1845836-1846150 [Pectobacterium parvum]
ATGCAATTCACCGTATACGGTAATACTGGGAAAAGTGTCGTTTACCCGCTGCTGCTCGATGTCACGAGCGATATTATTAG
ACAATTGAATCGTCGGATAGTGATCCCATTACTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCGTCATGCTGACGGACGGCAAAGAATACGCGGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAAGCCCTGGGG
GCGGTGTTTTGTGATGCTTCGCAGTACCGTTCTCAGGTAAAGGCTGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15375.45 Da        Isoelectric Point: 4.7132

>AT206902 WP_045349893.1 NZ_CP077264:2092213-2092632 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT206902 NZ_CP102749:1845597-1845833 [Pectobacterium parvum]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACGGTGGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
GATTAACCTGAGTGCTACTCTGTCAACCGCCTTGGATGCGGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAGGCTATCGACGCATTAAATCGTTTTCATGATGAAAACGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q486

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q1H0

References