Detailed information of TA system TA206877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 124052..124806
• Replicon: chromosome
• Accession: NZ_CP077262
• Organism: Citrobacter pasteurii strain FDAARGOS 1424

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6L54_RS00645
• Protein ID: WP_040233077.1
• Coordinates: 124052..124363 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L54_RS00650
• Protein ID: WP_040233078.1
• Coordinates: 124360..124806 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L54_RS00615 (I6L54_00615)	119718..120620	+	903	WP_005121817.1	formate dehydrogenase O subunit beta	-
I6L54_RS00620 (I6L54_00620)	120617..121252	+	636	WP_040233076.1	formate dehydrogenase cytochrome b556 subunit	-
I6L54_RS00625 (I6L54_00625)	121249..122178	+	930	WP_005121819.1	formate dehydrogenase accessory protein FdhE	-
I6L54_RS00630 (I6L54_00630)	122215..122589	-	375	WP_005121821.1	type II toxin-antitoxin system VapC family toxin	-
I6L54_RS00635 (I6L54_00635)	122589..122831	-	243	WP_005121822.1	CopG family transcriptional regulator	-
I6L54_RS00640 (I6L54_00640)	123037..123966	+	930	WP_005121823.1	alpha/beta hydrolase	-
I6L54_RS00645 (I6L54_00645)	124052..124363	+	312	WP_040233077.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6L54_RS00650 (I6L54_00650)	124360..124806	+	447	WP_040233078.1	helix-turn-helix domain-containing protein	Antitoxin
I6L54_RS00655 (I6L54_00655)	124818..125759	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
I6L54_RS00660 (I6L54_00660)	125804..126241	-	438	WP_003028671.1	D-aminoacyl-tRNA deacylase	-
I6L54_RS00665 (I6L54_00665)	126238..127110	-	873	WP_040233081.1	virulence factor BrkB family protein	-
I6L54_RS00670 (I6L54_00670)	127104..127703	-	600	WP_040233083.1	glucose-1-phosphatase	-
I6L54_RS00675 (I6L54_00675)	127809..128612	-	804	WP_003847907.1	DeoR/GlpR family DNA-binding transcription regulator	-
I6L54_RS00680 (I6L54_00680)	128646..129542	-	897	WP_040233085.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12533.52 Da
• Isoelectric Point: 9.2033

>T206877 WP_040233077.1 NZ_CP077262:124052-124363 [Citrobacter pasteurii]
VHVISRKPFNEGMLMYPNHGLALSELLHVLEKKTFHHPEEMKQYIPSLDNFKYRNKWWVIDVSGNNLRLIAYIDFRLHKV
FVKHIVLHAEYDKLTEYYRDHKE

Nucleotide

• Length: 312 bp

>T206877 NZ_CP102740:78900-79175 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16685.08 Da
• Isoelectric Point: 7.0634

>AT206877 WP_040233078.1 NZ_CP077262:124360-124806 [Citrobacter pasteurii]
MRSHRHMDAASAKKIVDTFSCAVKTIPLLGEERNETEYRRALALVEFLVDHDDLENPLFELLSARISEYEKHAPEFRALN
QHLKQIPSGVSVLRTLMDQYGLKATDLANELGSKSNVSNILNGRRALTVSHIKALAERFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT206877 NZ_CP102740:78616-78900 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAAGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGAAAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA