Detailed information of TA system TA206865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 317086..317734
• Replicon: chromosome
• Accession: NZ_CP077259
• Organism: Streptococcus mitis strain FDAARGOS 1456

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V8IC77
• Locus tag: I6L86_RS01390
• Protein ID: WP_000556942.1
• Coordinates: 317369..317734 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V8I9N0
• Locus tag: I6L86_RS01385
• Protein ID: WP_000797746.1
• Coordinates: 317086..317379 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L86_RS01375 (I6L86_01375)	313342..314208	-	867	WP_000089956.1	methylenetetrahydrofolate reductase [NAD(P)H]	-
I6L86_RS01380 (I6L86_01380)	314312..316561	-	2250	WP_000108228.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-
I6L86_RS01385 (I6L86_01385)	317086..317379	-	294	WP_000797746.1	helix-turn-helix transcriptional regulator	Antitoxin
I6L86_RS01390 (I6L86_01390)	317369..317734	-	366	WP_000556942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L86_RS01395 (I6L86_01395)	317890..318255	-	366	WP_001241814.1	TIGR02328 family protein	-
I6L86_RS01400 (I6L86_01400)	318239..318643	-	405	WP_000527884.1	YbgA family protein	-
I6L86_RS01405 (I6L86_01405)	318711..319412	-	702	WP_000748126.1	hypothetical protein	-
I6L86_RS01410 (I6L86_01410)	319547..320935	-	1389	WP_115262365.1	hypothetical protein	-
I6L86_RS01415 (I6L86_01415)	320932..321999	-	1068	WP_000415506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14545.72 Da
• Isoelectric Point: 9.3143

>T206865 WP_000556942.1 NZ_CP077259:c317734-317369 [Streptococcus mitis]
VHTIYFYKDKNGNEPVLDYMRELACRKSKDSRIKLNKLNDYIELLSQQGTRAGEPYMKHLEDEIWELRPLRDRILFVAWL
DGSFVLLHHFVKKTQKTPRREIEKAKRELKDLKERGVSDEE

Nucleotide

• Length: 366 bp

>T206865 NZ_CP102739:542683-543174 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10765.46 Da
• Isoelectric Point: 7.0172

>AT206865 WP_000797746.1 NZ_CP077259:c317379-317086 [Streptococcus mitis]
MKNSAIGSNWKDVRSELFTKEEILESDMRVAIMSELIEARHEQGISQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQRKS

Nucleotide

• Length: 294 bp

>AT206865 NZ_CP102739:542393-542686 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: V8IC77

Antitoxin

• AlphaFold DB: V8I9N0