Detailed information of TA system TA206842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 442947..443626
• Replicon: chromosome
• Accession: NZ_CP077242
• Organism: Escherichia fergusonii strain FDAARGOS 1438

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: I6L68_RS01975
• Protein ID: WP_002431667.1
• Coordinates: 443324..443626 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6L68_RS01970
• Protein ID: WP_000806441.1
• Coordinates: 442947..443288 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L68_RS01960 (438733)	438733..440022	-	1290	WP_000102764.1	APC family permease	-
I6L68_RS01965 (440329)	440329..442833	+	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
I6L68_RS01970 (442947)	442947..443288	-	342	WP_000806441.1	HigA family addiction module antitoxin	Antitoxin
I6L68_RS01975 (443324)	443324..443626	-	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6L68_RS01980 (443759)	443759..444553	+	795	WP_000365145.1	TraB/GumN family protein	-
I6L68_RS01985 (444757)	444757..445236	+	480	WP_000186628.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
I6L68_RS01990 (445260)	445260..446060	+	801	WP_000439795.1	hypothetical protein	-
I6L68_RS01995 (446057)	446057..446560	+	504	WP_000667001.1	hypothetical protein	-
I6L68_RS02000 (446598)	446598..448250	-	1653	WP_000670595.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T206842 WP_002431667.1 NZ_CP077242:c443626-443324 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T206842 NZ_CP102697:22226-22642 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT206842 WP_000806441.1 NZ_CP077242:c443288-442947 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT206842 NZ_CP102697:21999-22229 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8