Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 5032477..5033126 Replicon chromosome
Accession NZ_CP077211
Organism Enterobacter cloacae strain FDAARGOS 1431

Toxin (Protein)


Gene name higB Uniprot ID A0A5E1A383
Locus tag I6L61_RS24340 Protein ID WP_013097692.1
Coordinates 5032477..5032839 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5E1A3P3
Locus tag I6L61_RS24345 Protein ID WP_013097693.1
Coordinates 5032827..5033126 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L61_RS24320 (I6L61_24320) 5027811..5028782 - 972 WP_013097688.1 LacI family DNA-binding transcriptional regulator -
I6L61_RS24325 (I6L61_24325) 5028858..5030000 - 1143 WP_013097689.1 Gfo/Idh/MocA family oxidoreductase -
I6L61_RS24330 (I6L61_24330) 5029993..5031018 - 1026 WP_013097690.1 sugar phosphate isomerase/epimerase -
I6L61_RS24335 (I6L61_24335) 5031034..5032272 - 1239 WP_013097691.1 MFS transporter -
I6L61_RS24340 (I6L61_24340) 5032477..5032839 + 363 WP_013097692.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6L61_RS24345 (I6L61_24345) 5032827..5033126 + 300 WP_013097693.1 helix-turn-helix transcriptional regulator Antitoxin
I6L61_RS24350 (I6L61_24350) 5033252..5034640 - 1389 WP_013097694.1 phenylalanine transporter -
I6L61_RS24355 (I6L61_24355) 5034743..5036716 - 1974 WP_013097695.1 PhoX family phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5022588..5033126 10538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13994.00 Da        Isoelectric Point: 7.3235

>T206809 WP_013097692.1 NZ_CP077211:5032477-5032839 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Download         Length: 363 bp

>T206809 NZ_CP102690:c27686-27270 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10875.62 Da        Isoelectric Point: 8.6152

>AT206809 WP_013097693.1 NZ_CP077211:5032827-5033126 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Download         Length: 300 bp

>AT206809 NZ_CP102690:c27913-27683 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A383

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1A3P3

References