Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4576421..4577023 Replicon chromosome
Accession NZ_CP077206
Organism Enterobacter bugandensis strain FDAARGOS 1427

Toxin (Protein)


Gene name higB Uniprot ID A0A167J774
Locus tag I6L57_RS21730 Protein ID WP_047366784.1
Coordinates 4576712..4577023 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A167J796
Locus tag I6L57_RS21725 Protein ID WP_047366785.1
Coordinates 4576421..4576711 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L57_RS21710 (I6L57_21710) 4573919..4574821 + 903 WP_003861960.1 formate dehydrogenase subunit beta -
I6L57_RS21715 (I6L57_21715) 4574818..4575453 + 636 WP_015569914.1 formate dehydrogenase cytochrome b556 subunit -
I6L57_RS21720 (I6L57_21720) 4575450..4576379 + 930 WP_063437456.1 formate dehydrogenase accessory protein FdhE -
I6L57_RS21725 (I6L57_21725) 4576421..4576711 - 291 WP_047366785.1 NadS family protein Antitoxin
I6L57_RS21730 (I6L57_21730) 4576712..4577023 - 312 WP_047366784.1 hypothetical protein Toxin
I6L57_RS21735 (I6L57_21735) 4577172..4578113 - 942 WP_047366783.1 fatty acid biosynthesis protein FabY -
I6L57_RS21740 (I6L57_21740) 4578158..4578595 - 438 WP_008501836.1 D-aminoacyl-tRNA deacylase -
I6L57_RS21745 (I6L57_21745) 4578592..4579473 - 882 WP_045260235.1 virulence factor BrkB family protein -
I6L57_RS21750 (I6L57_21750) 4579467..4580066 - 600 WP_045260234.1 glucose-1-phosphatase -
I6L57_RS21755 (I6L57_21755) 4580154..4580624 - 471 WP_028014878.1 hypothetical protein -
I6L57_RS21760 (I6L57_21760) 4580621..4581364 - 744 WP_048959977.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12135.13 Da        Isoelectric Point: 8.8415

>T206790 WP_047366784.1 NZ_CP077206:c4577023-4576712 [Enterobacter bugandensis]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPEYGDVIQNTGGLRKIRWLAGGKGKRGGVRVIYFYRTCEFEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Download         Length: 312 bp

>T206790 NZ_CP102679:4899840-4900115 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10674.22 Da        Isoelectric Point: 10.5888

>AT206790 WP_047366785.1 NZ_CP077206:c4576711-4576421 [Enterobacter bugandensis]
MDKALFERLTQSVGQMNEIAEGQREPSRIFQIDAMKIKEIRQASGLSQSKFATLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Download         Length: 291 bp

>AT206790 NZ_CP102679:4900112-4900471 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167J774

Antitoxin

Source ID Structure
AlphaFold DB A0A167J796

References