Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6066424..6067150 Replicon chromosome
Accession NZ_CP077093
Organism Pseudomonas vanderleydeniana strain RW8P3

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HU752_RS27275 Protein ID WP_186678239.1
Coordinates 6066839..6067150 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A9E6PJC5
Locus tag HU752_RS27270 Protein ID WP_017902440.1
Coordinates 6066424..6066837 (-) Length 138 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU752_RS27250 (HU752_027250) 6061948..6063597 - 1650 WP_186678221.1 alpha,alpha-phosphotrehalase -
HU752_RS27255 (HU752_027255) 6063629..6065071 - 1443 WP_186678235.1 PTS system trehalose-specific EIIBC component -
HU752_RS27260 (HU752_027260) 6065296..6066000 + 705 WP_186678237.1 trehalose operon repressor -
HU752_RS27265 (HU752_027265) 6066020..6066310 - 291 WP_225920075.1 hypothetical protein -
HU752_RS27270 (HU752_027270) 6066424..6066837 - 414 WP_017902440.1 transcriptional regulator Antitoxin
HU752_RS27275 (HU752_027275) 6066839..6067150 - 312 WP_186678239.1 type II toxin-antitoxin system HigB family toxin Toxin
HU752_RS27280 (HU752_027280) 6067447..6069024 - 1578 WP_186678241.1 glutamine-hydrolyzing GMP synthase -
HU752_RS27285 (HU752_027285) 6069130..6070599 - 1470 WP_054057407.1 IMP dehydrogenase -
HU752_RS27290 (HU752_027290) 6070682..6071230 - 549 WP_186678242.1 sugar ABC transporter ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12154.85 Da        Isoelectric Point: 9.3209

>T206734 WP_186678239.1 NZ_CP077093:c6067150-6066839 [Pseudomonas vanderleydeniana]
MHVISRKPFSDAASAYPNDVEAIDRTYRVLRSGEFDTPQALGKVFPSLDNFKYKDKWWVLDIGGNNLRLIAFIEFRDNRL
YVKHIVTHAEYDKLCKRYRKEAD

Download         Length: 312 bp

>T206734 NZ_CP102672:c35288-35034 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 138 a.a.        Molecular weight: 15111.29 Da        Isoelectric Point: 4.8002

>AT206734 WP_017902440.1 NZ_CP077093:c6066837-6066424 [Pseudomonas vanderleydeniana]
MGFAAIKEKAYALFSEAGFISHIANDDDYAKALALMDDLVEDYEVNRPLIEILARSIESWENESEEFVAFNARVAKLGGV
DVLRLLMEQHGLGVADLPEIGSKSLVSKILNGRGRNLTRDHIAALSKRFAVSPALFF

Download         Length: 414 bp

>AT206734 NZ_CP102672:c35037-34795 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References