Detailed information of TA system TA206660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6037501..6038092
• Replicon: chromosome
• Accession: NZ_CP077081
• Organism: Pseudomonas atacamensis strain SWRI76

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KSS92_RS26855
• Protein ID: WP_082228595.1
• Coordinates: 6037814..6038092 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KSS92_RS26850
• Protein ID: WP_217879001.1
• Coordinates: 6037501..6037806 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS92_RS26820 (KSS92_26820)	6033053..6033760	+	708	WP_217879000.1	HAD family hydrolase	-
KSS92_RS27335	6033770..6033871	-	102	Protein_5318	metal ABC transporter ATP-binding protein	-
KSS92_RS26825 (KSS92_26825)	6033950..6034276	-	327	WP_016772583.1	transcriptional regulator SutA	-
KSS92_RS26830 (KSS92_26830)	6034378..6034803	-	426	WP_071172132.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
KSS92_RS26835 (KSS92_26835)	6035019..6036356	-	1338	WP_041476939.1	ammonium transporter	-
KSS92_RS26840 (KSS92_26840)	6036394..6036732	-	339	WP_002555808.1	P-II family nitrogen regulator	-
KSS92_RS26845 (KSS92_26845)	6037127..6037387	+	261	WP_122603430.1	accessory factor UbiK family protein	-
KSS92_RS26850 (KSS92_26850)	6037501..6037806	-	306	WP_217879001.1	HigA family addiction module antitoxin	Antitoxin
KSS92_RS26855 (KSS92_26855)	6037814..6038092	-	279	WP_082228595.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KSS92_RS26860 (KSS92_26860)	6038419..6039261	+	843	Protein_5326	ATP-binding protein	-
KSS92_RS26865 (KSS92_26865)	6040183..6040959	+	777	WP_225914397.1	GAF domain-containing protein	-
KSS92_RS26870 (KSS92_26870)	6040959..6041390	+	432	WP_217879002.1	hypothetical protein	-
KSS92_RS26875 (KSS92_26875)	6041468..6042499	-	1032	WP_217879003.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10401.95 Da
• Isoelectric Point: 7.7181

>T206660 WP_082228595.1 NZ_CP077081:c6038092-6037814 [Pseudomonas atacamensis]
MIVSFRCPETEYLFRSGKTRFWSAILSVAERKLTMLDAAAALADLRSPPGNRLESLGGDRKGQCSIRINAQWRICFVWGL
NGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T206660 NZ_CP102552:354216-354563 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11262.99 Da
• Isoelectric Point: 6.4556

>AT206660 WP_217879001.1 NZ_CP077081:c6037806-6037501 [Pseudomonas atacamensis]
MTKNGMRPVHPGEILKEEYLEPLSLTAAALARALSVSTPTVNDIVLQRRGISADMALRLSICLDTTPEFWLNLQSTYDLR
KAEIERGTAIRDQVERLAHCA

Nucleotide

• Length: 306 bp

>AT206660 NZ_CP102552:354563-354862 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA