Detailed information of TA system TA206658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5413317..5413897
• Replicon: chromosome
• Accession: NZ_CP077081
• Organism: Pseudomonas atacamensis strain SWRI76

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1E8P922
• Locus tag: KSS92_RS23900
• Protein ID: WP_038861219.1
• Coordinates: 5413317..5413595 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KSS92_RS23905
• Protein ID: WP_016773085.1
• Coordinates: 5413610..5413897 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KSS92_RS23880 (KSS92_23880)	5408803..5408970	-	168	WP_016773090.1	DUF2474 domain-containing protein	-
KSS92_RS23885 (KSS92_23885)	5409055..5410062	-	1008	WP_016773089.1	cytochrome d ubiquinol oxidase subunit II	-
KSS92_RS23890 (KSS92_23890)	5410066..5411511	-	1446	WP_024014409.1	cytochrome ubiquinol oxidase subunit I	-
KSS92_RS23895 (KSS92_23895)	5411982..5413217	+	1236	WP_206421530.1	MFS transporter	-
KSS92_RS23900 (KSS92_23900)	5413317..5413595	+	279	WP_038861219.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KSS92_RS23905 (KSS92_23905)	5413610..5413897	+	288	WP_016773085.1	HigA family addiction module antitoxin	Antitoxin
KSS92_RS23910 (KSS92_23910)	5413902..5414453	-	552	WP_217878856.1	DJ-1/PfpI family protein	-
KSS92_RS23915 (KSS92_23915)	5414521..5414967	-	447	WP_125918175.1	DUF4879 domain-containing protein	-
KSS92_RS23920 (KSS92_23920)	5415188..5416483	+	1296	WP_016773083.1	NCS2 family permease	-
KSS92_RS23925 (KSS92_23925)	5416480..5417559	+	1080	WP_217878857.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
KSS92_RS23930 (KSS92_23930)	5417614..5418060	-	447	WP_217878858.1	DUF2442 domain-containing protein	-
KSS92_RS23935 (KSS92_23935)	5418353..5418793	+	441	WP_016773081.1	type II 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10757.32 Da
• Isoelectric Point: 9.5056

>T206658 WP_038861219.1 NZ_CP077081:5413317-5413595 [Pseudomonas atacamensis]
MIKSFQHKGLRGFYETGTTRGIRADHAKRLSRMLQFMDRATLPGDLDLPGWRLHPLKGELSEYWSLSVSGNWRVIFRFVG
SDIELVDYLDYH

Nucleotide

• Length: 279 bp

>T206658 NZ_CP102548:116815-117195 [Klebsiella pneumoniae]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10525.13 Da
• Isoelectric Point: 6.6962

>AT206658 WP_016773085.1 NZ_CP077081:5413610-5413897 [Pseudomonas atacamensis]
MPMHNPPHPGETLLLDVLPELGISVSELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARVWLGVQTDYDLWQA
EHREQPMIEPLAAHG

Nucleotide

• Length: 288 bp

>AT206658 NZ_CP102548:116594-116815 [Klebsiella pneumoniae]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A1E8P922