Detailed information of TA system TA206589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2556473..2557070
• Replicon: chromosome
• Accession: NZ_CP076863
• Organism: Klebsiella pneumoniae strain V3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KRP25_RS12455
• Protein ID: WP_217048960.1
• Coordinates: 2556753..2557070 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KRP25_RS12450
• Protein ID: WP_004142561.1
• Coordinates: 2556473..2556760 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRP25_RS12420 (2552553)	2552553..2552801	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
KRP25_RS12425 (2552819)	2552819..2553160	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KRP25_RS12430 (2553191)	2553191..2554306	-	1116	WP_217048956.1	MBL fold metallo-hydrolase	-
KRP25_RS12435 (2554486)	2554486..2555067	+	582	WP_217048957.1	TetR/AcrR family transcriptional regulator	-
KRP25_RS12440 (2555067)	2555067..2555435	+	369	WP_217048958.1	MmcQ/YjbR family DNA-binding protein	-
KRP25_RS12445 (2555555)	2555555..2556208	+	654	WP_217048959.1	oxygen-insensitive NAD(P)H nitroreductase	-
KRP25_RS12450 (2556473)	2556473..2556760	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KRP25_RS12455 (2556753)	2556753..2557070	-	318	WP_217048960.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KRP25_RS12460 (2557255)	2557255..2558298	-	1044	WP_217048961.1	DUF2157 domain-containing protein	-
KRP25_RS12465 (2558968)	2558968..2559834	-	867	WP_217048962.1	helix-turn-helix transcriptional regulator	-
KRP25_RS12470 (2559943)	2559943..2561370	+	1428	WP_217048963.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12088.38 Da
• Isoelectric Point: 11.4448

>T206589 WP_217048960.1 NZ_CP076863:c2557070-2556753 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVRAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLILLRVF
VKKTQKTPSSEIRPALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T206589 NZ_CP102490:c4924028-4923111 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCTAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT206589 WP_004142561.1 NZ_CP076863:c2556760-2556473 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT206589 NZ_CP102490:c4924792-4924034 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1