Detailed information of TA system TA206569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2088280..2088911
• Replicon: chromosome
• Accession: NZ_CP076853
• Organism: Rhizobium sp. WYJ-E13

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KQ933_RS10515
• Protein ID: WP_216758712.1
• Coordinates: 2088627..2088911 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KQ933_RS10510
• Protein ID: WP_216758711.1
• Coordinates: 2088280..2088579 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ933_RS10480 (KQ933_10480)	2083548..2084894	+	1347	WP_216758708.1	carbohydrate porin	-
KQ933_RS10485 (KQ933_10485)	2084928..2085815	-	888	WP_216758881.1	hypothetical protein	-
KQ933_RS10490 (KQ933_10490)	2086133..2086348	+	216	WP_216758709.1	hypothetical protein	-
KQ933_RS10495 (KQ933_10495)	2086430..2087221	+	792	WP_183735808.1	potassium channel family protein	-
KQ933_RS10500 (KQ933_10500)	2087268..2087732	+	465	Protein_2079	substrate-binding domain-containing protein	-
KQ933_RS10505 (KQ933_10505)	2087905..2088042	+	138	WP_216758710.1	hypothetical protein	-
KQ933_RS10510 (KQ933_10510)	2088280..2088579	-	300	WP_216758711.1	HigA family addiction module antitoxin	Antitoxin
KQ933_RS10515 (KQ933_10515)	2088627..2088911	-	285	WP_216758712.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQ933_RS10520 (KQ933_10520)	2089022..2089762	-	741	WP_216758713.1	phosphatase PAP2 family protein	-
KQ933_RS10525 (KQ933_10525)	2090069..2090761	+	693	WP_216758714.1	response regulator	-
KQ933_RS10530 (KQ933_10530)	2090758..2091927	+	1170	WP_216758715.1	ATP-binding protein	-
KQ933_RS10535 (KQ933_10535)	2091949..2092899	-	951	WP_253958315.1	DUF535 family protein	-
KQ933_RS10540 (KQ933_10540)	2093294..2093533	+	240	WP_216758717.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-94)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-95)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10511.02 Da
• Isoelectric Point: 8.0808

>T206569 WP_216758712.1 NZ_CP076853:c2088911-2088627 [Rhizobium sp. WYJ-E13]
VIRSFKTAMTETVATTQKAPKGFPADLMRVAQRKLAMLNAAAVLDDLRSPPGNRLEALKGNRQGQHSIRIDDQWRICFVW
TEAGAEDVEIVDHH

Nucleotide

• Length: 285 bp

>T206569 NZ_CP102488:3259951-3260328 [Escherichia coli O15:H18]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11054.70 Da
• Isoelectric Point: 5.1373

>AT206569 WP_216758711.1 NZ_CP076853:c2088579-2088280 [Rhizobium sp. WYJ-E13]
MLKILTPVHPGEILREEYLKPLALSAGALARKLNVPRTRIERLASEQTPVTTDTALRLAKYFRTTPDFWLNLQSSYDLGV
EAPALQEELDAIEEIGHAA

Nucleotide

• Length: 300 bp

>AT206569 NZ_CP102488:3259527-3259904 [Escherichia coli O15:H18]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA