Detailed information of TA system TA206558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 213986..214622
• Replicon: chromosome
• Accession: NZ_CP076847
• Organism: Agrobacterium pusense strain SX41

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A098RNJ2
• Locus tag: KP800_RS15885
• Protein ID: WP_006697304.1
• Coordinates: 214341..214622 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KP800_RS15880
• Protein ID: WP_004444714.1
• Coordinates: 213986..214285 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP800_RS15855 (KP800_15855)	209172..209900	-	729	WP_037092566.1	ABC transporter ATP-binding protein	-
KP800_RS15860 (KP800_15860)	209897..210805	-	909	WP_037092567.1	branched-chain amino acid ABC transporter permease	-
KP800_RS15865 (KP800_15865)	210802..211674	-	873	WP_037092568.1	branched-chain amino acid ABC transporter permease	-
KP800_RS15870 (KP800_15870)	211745..212959	-	1215	WP_037092569.1	amino acid ABC transporter substrate-binding protein	-
KP800_RS15875 (KP800_15875)	213092..213799	+	708	WP_234797374.1	hypothetical protein	-
KP800_RS26750	213709..213966	+	258	WP_234797373.1	helix-turn-helix transcriptional regulator	-
KP800_RS15880 (KP800_15880)	213986..214285	-	300	WP_004444714.1	HigA family addiction module antitoxin	Antitoxin
KP800_RS15885 (KP800_15885)	214341..214622	-	282	WP_006697304.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KP800_RS15890 (KP800_15890)	214697..215506	-	810	WP_037092570.1	ABC transporter ATP-binding protein	-
KP800_RS15895 (KP800_15895)	215503..216471	-	969	WP_037092571.1	iron chelate uptake ABC transporter family permease subunit	-
KP800_RS15900 (KP800_15900)	216474..217517	-	1044	WP_037092572.1	iron ABC transporter permease	-
KP800_RS15905 (KP800_15905)	217514..218521	-	1008	WP_037092598.1	iron-siderophore ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10536.95 Da
• Isoelectric Point: 9.0298

>T206558 WP_006697304.1 NZ_CP076847:c214622-214341 [Agrobacterium pusense]
MIRSFKNKLAASIDDGSVKKGFPSDMLRRAQQLLTVLDAATTVEDPRSPPGNRLEKLSGDREGQYSIRINKQWRICFVWT
QAGPESVEITDYH

Nucleotide

• Length: 282 bp

>T206558 NZ_CP102488:1069948-1071270 [Escherichia coli O15:H18]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACTGTCGCTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11060.86 Da
• Isoelectric Point: 8.5148

>AT206558 WP_004444714.1 NZ_CP076847:c214285-213986 [Agrobacterium pusense]
MTISKSPPIHPGEILRELYLEPLGMSAYALARHLNVPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
AAAARLETDLANIKELEAA

Nucleotide

• Length: 300 bp

>AT206558 NZ_CP102488:1069682-1069948 [Escherichia coli O15:H18]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGTATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A098RNJ2