Detailed information of TA system TA206484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4524..5109
• Replicon: plasmid p2UC20804
• Accession: NZ_CP076809
• Organism: Acinetobacter baumannii strain UC20804

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: KQ254_RS19565
• Protein ID: WP_000897309.1
• Coordinates: 4524..4844 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A009MRQ1
• Locus tag: KQ254_RS19570
• Protein ID: WP_032001914.1
• Coordinates: 4837..5109 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ254_RS19530 (KQ254_19430)	1..924	+	924	WP_033107831.1	replication initiation protein RepM	-
KQ254_RS19535 (KQ254_19435)	1009..1542	+	534	WP_033107830.1	plasmid replication DNA-binding protein	-
KQ254_RS19540 (KQ254_19440)	1829..2356	+	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
KQ254_RS19545 (KQ254_19445)	2537..3148	+	612	WP_008942536.1	recombinase family protein	-
KQ254_RS19550 (KQ254_19450)	3533..3748	+	216	WP_000071892.1	cold-shock protein	-
KQ254_RS19555 (KQ254_19455)	3846..4073	+	228	WP_000921865.1	hypothetical protein	-
KQ254_RS19560 (KQ254_19460)	4140..4337	+	198	WP_000476222.1	hypothetical protein	-
KQ254_RS19565 (KQ254_19465)	4524..4844	+	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQ254_RS19570 (KQ254_19470)	4837..5109	+	273	WP_032001914.1	NadS family protein	Antitoxin
KQ254_RS19575 (KQ254_19475)	5583..6188	-	606	WP_000447788.1	LysE family translocator	-
KQ254_RS19580 (KQ254_19480)	6238..7065	-	828	WP_000073658.1	AraC family transcriptional regulator	-
KQ254_RS19585 (KQ254_19485)	7239..7945	-	707	Protein_11	IS1 family transposase	-
KQ254_RS19590 (KQ254_19490)	8036..8878	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
KQ254_RS19595 (KQ254_19495)	9083..9787	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KQ254_RS19600 (KQ254_19500)	9821..9982	-	162	WP_153603871.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-58	-	1..17469	17469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T206484 WP_000897309.1 NZ_CP076809:4524-4844 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T206484 NZ_CP102441:c176383-176102 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10088.61 Da
• Isoelectric Point: 8.6582

>AT206484 WP_032001914.1 NZ_CP076809:4837-5109 [Acinetobacter baumannii]
MDNNLLDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT206484 NZ_CP102441:c176607-176380 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3

Antitoxin

• AlphaFold DB: A0A009MRQ1