Detailed information of TA system TA206479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3245..3896
• Replicon: plasmid p2UC25604
• Accession: NZ_CP076803
• Organism: Acinetobacter baumannii strain UC25604

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: KQ252_RS18975
• Protein ID: WP_000269904.1
• Coordinates: 3245..3601 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQ252_RS18980
• Protein ID: WP_001129974.1
• Coordinates: 3594..3896 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ252_RS18960 (KQ252_18910)	1..930	+	930	WP_005133531.1	replication initiation protein RepM	-
KQ252_RS18965 (KQ252_18915)	1009..1533	+	525	WP_005127114.1	plasmid replication DNA-binding protein	-
KQ252_RS18970 (KQ252_18920)	1700..2629	-	930	WP_045900839.1	potassium channel family protein	-
KQ252_RS18975 (KQ252_18925)	3245..3601	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQ252_RS18980 (KQ252_18930)	3594..3896	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
KQ252_RS18985 (KQ252_18935)	3889..4098	+	210	WP_000069474.1	hypothetical protein	-
KQ252_RS18990 (KQ252_18940)	4424..4885	-	462	WP_000761346.1	DIP1984 family protein	-
KQ252_RS18995 (KQ252_18945)	5190..7601	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
KQ252_RS19000 (KQ252_18950)	7729..8043	-	315	WP_001983304.1	BrnA antitoxin family protein	-
KQ252_RS19005 (KQ252_18955)	8030..8317	-	288	WP_000438826.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11203	11203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T206479 WP_000269904.1 NZ_CP076803:3245-3601 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T206479 NZ_CP102438:c5119420-5119037 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCAGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT206479 WP_001129974.1 NZ_CP076803:3594-3896 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT206479 NZ_CP102438:c5119662-5119420 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: N8YFZ1