Detailed information of TA system TA020645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1147310..1147928
• Replicon: chromosome
• Accession: NC_003047
• Organism: Sinorhizobium meliloti 1021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: SM_RS05365
• Protein ID: WP_010969011.1
• Coordinates: 1147632..1147928 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SM_RS05360
• Protein ID: WP_010969010.1
• Coordinates: 1147310..1147618 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM_RS05325 (SMc02387)	1142389..1142709	-	321	WP_003527320.1	YnfA family protein	-
SM_RS31255 (SMc02388)	1142784..1142957	-	174	WP_010969006.1	hypothetical protein	-
SM_RS31260 (SMc02389)	1142971..1143144	-	174	WP_003527318.1	hypothetical protein	-
SM_RS05330 (SMc02390)	1143380..1144081	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
SM_RS05335 (SMc02391)	1144305..1144646	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
SM_RS05340 (SMc02392)	1145231..1145497	+	267	WP_003527312.1	sel1 repeat family protein	-
SM_RS05345 (SMc02393)	1145519..1146640	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
SM_RS05355	1147069..1147299	-	231	WP_026029805.1	hypothetical protein	-
SM_RS05360 (SMc02394)	1147310..1147618	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
SM_RS05365 (SMc02461)	1147632..1147928	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SM_RS05370	1147976..1148281	-	306	WP_003527305.1	hypothetical protein	-
SM_RS05375 (SMc02396)	1148609..1149646	+	1038	WP_003527304.1	porin	-
SM_RS05380 (SMc02397)	1149756..1150823	-	1068	WP_014526650.1	site-specific integrase	-
SM_RS05385 (SMc02411)	1150950..1151896	+	947	WP_011970797.1	IS630-like element ISRm2011-2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T20645 WP_010969011.1 NC_003047:c1147928-1147632 [Sinorhizobium meliloti 1021]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T20645 NC_003047:c1147928-1147632 [Sinorhizobium meliloti 1021]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT20645 WP_010969010.1 NC_003047:c1147618-1147310 [Sinorhizobium meliloti 1021]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT20645 NC_003047:c1147618-1147310 [Sinorhizobium meliloti 1021]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61